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Strain-Level Metagenomic Data Analysis of Enriched In Vitro and In Silico Spiked Food Samples: Paving the Way towards a Culture-Free Foodborne Outbreak Investigation Using STEC as a Case Study.
simulated metagenomic data to explore the possibilities and limitations of this data analysis approach. The performed analysis allowed us to link the pathogenic strains from food samples to human isolates ...
Global burden of late-stage chronic kidney disease resulting from dietary exposure to cadmium, 2015
performed to estimate the Disability-Adjusted Life Years (DALY s) due to late-stage CKD associated with dietary exposures to cadmium. Using the distribution of population GFR s, the prevalence of CKD was ...
Use of next‐generation sequencing in microbial risk assessment
hazard characterisation and exposure assessment to perform a sound risk characterisation, which in turn will make it possible to take substantiated risk management decisions. Health Topics: Food-borne ...
Growth of Stressed Strains of Four Non-O157 Shiga Toxin-Producing Escherichia coli Serogroups in Five Enrichment Broths.
dynamics. The two selective media, Brila and SEB, were less efficient than BPW, but Brila’s enrichment performance was remarkably better than that of SEB. This study shows that irrespective of the effect of ...
Seroprevalence of zoonotic parasites in pigs slaughtered in the Kathmandu Valley of Nepal
For several years, the demand for pork has been on the rise in Nepal. To assess the importance of pork as a carrier of zoonotic agents, we performed a cross-sectional study in the Kathmandu Valley of ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates.
confounding factors such as EDTA in DNA solution buffers. Nevertheless, completeness of STEC characterization was, although not substantially, influenced by the plasmid extraction performance of the kits, ...