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A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.

chain reactions (qPCRs) are used in routine. In recent years, the use of whole genome sequencing (WGS) of bacterial isolates has proven its value to collect relevant information for strain ...

Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.

Nucleotide Real-Time Polymerase Chain Reaction RNA Replicase RNA, Viral Sensitivity and Specificity whole genome sequencing Abstract: The current CO ronaVIrus Disease 2019 (COVID-19) pandemic started in ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses

outbreak, early diagnosis, and therefore fast and adequate detection, is needed. To this end, a multiplex reverse transcription real-time polymerase chain reaction TaqMan method was designed to detect Zika ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.

a multiplex reverse transcription real-time polymerase chain reaction TaqMan® method was designed to detect universally Zika and chikungunya viruses. DESIGN: Two methods, targeting different genome segments, ...

Study of the underlying mechanisms and consequences of pathogenicity differences between two in vitro selected G1-H9N2 clones originating from a single isolate.

Influenza in Birds Poultry Diseases Real-Time Polymerase Chain Reaction Sequence Analysis, DNA Viral Plaque Assay virulence Virus Shedding Abstract: The G1- H9N2 avian influenza virus (AIV) has caused ...

Seroprevalence of border disease virus and other pestiviruses in sheep in Algeria and associated risk factors.

Issue 1 (2018) Keywords: Algeria Animals Border Disease Border disease virus Enzyme-Linked Immunosorbent Assay Female Male Pestivirus Pestivirus Infections Real-Time Polymerase Chain Reaction Risk Factors ...

Orthogonal typing methods identify genetic diversity among Belgian Campylobacter jejuni strains isolated over a decade from poultry and cases of sporadic human illness.

Campylobacter jejuni Chickens Gastroenteritis Genetic Variation Humans Molecular Epidemiology Multilocus Sequence Typing polymerase chain reaction Poultry Abstract: Campylobacter jejuni is a zoonotic pathogen ...

Hepatitis E virus serology and PCR: does the methodology matter?

Viral Hepatitis E Hepatitis E virus Humans Immunoglobulin G Immunoglobulin M polymerase chain reaction Sensitivity and Specificity Serologic Tests Abstract: Hepatitis E virus (HEV) genotype 3 is an ...

Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.

Keywords: 5' Untranslated Regions Animals DNA Primers Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sensitivity ...

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