Search results - 298 results

Whole Genome Sequencing Provides an Added Value to the Investigation of Staphylococcal Food Poisoning Outbreaks

poisoning (SFP) is mostly performed using immunoassays, which, however, only detect five of 27 SE s described to date. Polymerase chain reactions are, therefore, frequently used in complement to identify ...

A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.

outbreak depends on the rapid and accurate identification of the responsible food source. Conventional methods based on isolation of the pathogen from the food matrix and target-specific real-time polymerase ...

Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.

Polymorphism, Single Nucleotide Real-Time Polymerase Chain Reaction RNA Replicase RNA, Viral Sensitivity and Specificity whole genome sequencing Abstract: The current CO ronaVIrus Disease 2019 (COVID-19) pandemic ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses

early diagnosis, and therefore fast and adequate detection, is needed. To this end, a multiplex reverse transcription real-time polymerase chain reaction TaqMan method was designed to detect Zika (ZIKV) ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.

transcription real-time polymerase chain reaction TaqMan® method was designed to detect universally Zika and chikungunya viruses. DESIGN: Two methods, targeting different genome segments, were selected from ...

Study of the underlying mechanisms and consequences of pathogenicity differences between two in vitro selected G1-H9N2 clones originating from a single isolate.

Influenza in Birds Poultry Diseases Real-Time Polymerase Chain Reaction Sequence Analysis, DNA Viral Plaque Assay virulence Virus Shedding Abstract: The G1- H9N2 avian influenza virus (AIV) has caused ...

Detecting cervical precancer and reaching underscreened women by using HPV testing on self samples: updated meta-analyses.

participation trials were included. hrHPV assays based on polymerase chain reaction were as sensitive on self samples as on clinician samples to detect CIN2 + or CIN3 + (pooled ratio 0.99, 95% confidence interval ...

Seroprevalence of border disease virus and other pestiviruses in sheep in Algeria and associated risk factors.

Issue 1 (2018) Keywords: Algeria Animals Border Disease Border disease virus Enzyme-Linked Immunosorbent Assay Female Male Pestivirus Pestivirus Infections Real-Time Polymerase Chain Reaction Risk Factors ...

Orthogonal typing methods identify genetic diversity among Belgian Campylobacter jejuni strains isolated over a decade from poultry and cases of sporadic human illness.

Campylobacter jejuni Chickens Gastroenteritis Genetic Variation Humans Molecular Epidemiology Multilocus Sequence Typing polymerase chain reaction Poultry Abstract: Campylobacter jejuni is a zoonotic pathogen ...

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