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Selection and use of reference panels: a case study highlighting current gaps in the materials available for foot and mouth disease.

assays used to measure virus-specific FMD antibody responses. Since there are often limited resources to prepare and distribute these materials, sustainable progress in this arena will only be achievable ...

Review: Vaccines and Vaccination against Lumpy Skin Disease

vaccination against LSD, to better prepare affected and at-risk countries to control LSD and ensure the safe trade of cattle. Health Topics:  Animal health Santé animale Diergezondheid Service:  Exotic and ...

Selection and use of reference panels: a case study highlighting current gaps in the materials available for foot and mouth disease

post-vaccination serology, and address inter-laboratory differences in serological assays used to measure virus-specific FMD antibody responses. Since there are often limited resources to prepare and distribute ...

Transmission electron microscopy sample preparation protocols for the ultrastructural study of cysts of free-living protozoa.

however, conventional TEM protocols are not satisfactory for cysts of free-living protozoa. Here we report on the design and testing of four protocols for TEM sample preparation of cysts. Two protocols, one ...

Pre-registration efficacy study of a novel marker vaccine against classical swine fever on Maternally Derived Antibody negative (MDA-) target animals.

prepared in cell cultures), classical” 7th Edition, which claims the efficacy test is invalid if fewer than 50 per cent of the control piglets display typical signs of serious infection of CSF or die, and if ...

Rapid generation of replication-deficient monovalent and multivalent vaccines for bluetongue virus: protection against virulent virus challenge in cattle and sheep.

protection with a multivalent preparation of six distinct DISC viruses. These data indicate that a very-rapid-response vaccine could be developed based on which serotypes are circulating in the population at ...

The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing.

Independent Single Primer Amplification is one of the most widely used random amplification approaches in virology for sequencing template preparation. This technique relies on oligonucleotides consisting of ...

In vitro and in vivo infectious potential of coxiella burnetii: a study on Belgian livestock isolates.

in the Netherlands during the 2007-2010 outbreaks. Infective doses were established with ethidium monoazide- PCR for the first time here applied to C. burnetii. This method allowed for the preparation ...

Proteomic characterization of bovine herpesvirus 4 extracellular virions.

preparations allowed us to define protein localization. In parallel, in order to identify some previously undefined open reading frames, we mapped peptides detected in whole virion lysates onto the six frames of ...

NUSAP method for evaluating the data quality in a quantitative microbial risk assessment model for Salmonella in the pork production chain.

parameters were grouped according to four successive exposure pathways: (1) primary production (2) transport, holding, and slaughterhouse, (3) postprocessing, distribution, and storage, and (4) preparation and ...

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