Search results - 103 results
Efficient control of Japanese encephalitis virus in the central nervous system of infected pigs occurs in the absence of a pronounced inflammatory immune response.
JEV genotype 3 Nakayama strain. Clinical signs were assessed daily, and viral spread was followed by RT-qPCR. mRNA expression profiles were determined to study immune responses in the CNS. RESULTS: ...
QuilA-Adjuvanted T. gondii Lysate Antigens Trigger Robust Antibody and IFNγ+ T Cell Responses in Pigs Leading to Reduction in Parasite DNA in Tissues Upon Challenge InfectionImage
Immunology, Volume 10 (2019) Keywords: magnetic capture-qPCR Pigs QuilA TLA vaccine Toxoplasma gondii Abstract: Toxoplasma gondii is an intracellular parasite of all mammals and birds, responsible for ...
Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.
December 2019. COVID-19 cases are confirmed by the detection of SARS-CoV-2 RNA in biological samples by RT-qPCR. However, limited numbers of SARS-CoV-2 genomes were available when the first RT-qPCR methods ...
The African swine fever virus isolate Belgium 2018/1 shows high virulence in European wild boar.
severe disease course with typical pathomorphological and histopathological lesions. Organs and blood samples were positive in qPCR, haemadsorption test and antigen lateral flow devices (LFD). Virus and ...
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses
Dominique Rousset; Steven Van Gucht *; Nancy Roosens * Source: International Journal of Infectious Diseases, Volume 92 (2020) Keywords: Chikungunya virus discrimination identification multiplex RT-qPCR Zika ...
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.
Publication Type: Peer reviewed scientific article Authors: Broeders, Sylvia; Garlant, Linda; Fraiture, Marie-Alice; Vandermassen, Els; Suin, Vanessa; Vanhomwegen, Jessica; Dupont-Rouzeyrol, Myrielle; Rousset, Dominique; Steven Van Gucht; Roosens, Nancy S ...
Combining a genotoxin-specific qPCR method with the ToxTracker to evaluate the genotoxic potential of high priority printed paper and board food contact material substances
Publication Type: Scientific poster, presentation or proceeding Authors: Melissa Van Bossuyt; Hendriks G; Derr R; Doktorova TY; Van Hoeck E; Vanhaecke T; Rogiers V; Birgit Mertens Source: EEMGS meeting 2019, Rennes (France) (2019) Service: Évaluation des ...
Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study.
real-time polymerase chain reaction (RT-qPCR) directed against genomic regions conserved between different isolates. Genetic variation can however result in mismatches of primers and probes with their ...
Very Long Persistence of Botulinum Toxin B in a Patient’s Serum
immunocompromised adults) botulism. Case Report: A case of human botulism type B due to the consumption of an imported dried ham is reported. Laboratory results confirmed the diagnosis using the mouse bioassay and the qPCR ...
A novel genotoxin-specific qPCR array based on the metabolically competent human HepaRG™ cell line as a rapid and reliable tool for improved in vitro hazard assessment
Keersmaecker; Rogiers, Vera; Tatyana Y. Doktorova Source: Archives of Toxicology (2018) Keywords: genotoxicity HepaRG In vitro screening mutagenicity qPCR array Toxicogenomics Abstract: Although the value of the ...