Search results - 91 results

Pilot market surveillance of GMM contaminations in alpha-amylase food enzyme products: A detection strategy strengthened by a newly developed qPCR method targeting a GM Bacillus licheniformis producing alpha-amylase

Publication Type: Peer reviewed scientific article Authors: Fraiture, Marie-Alice; Gobbo, Andrea; Guillitte, Chloé; Marchesi, Ugo; Verginelli, Daniela; De Greve, Joke; D'aes, Jolien; Vanneste, Kevin; N. Papazova; Roosens, Nancy H.C. Source: Food Chem ...

Intra- and interlaboratory reproducibility of the RIATOL qPCR HPV genotyping assay.

compared to a standard comparator for cervical precancer and good reproducibility. This study builds upon previous diagnostic accuracy assessments of the RIATOL HPV genotyping qPCR assay and aims to evaluate ...

Risk Factors Associated with Severe RSV Infection in Infants: What Is the Role of Viral Co-Infections?

for a panel of 16 different respiratory viruses with multiplex RT-qPCR. Disease severity was assessed with traditional clinical parameters and scoring systems. A total of 120 patients were included, of ...

Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants.

existing reverse transcriptase real-time PCR (RT-qPCR) assay, we developed a reverse transcriptase droplet digital PCR assay (RT-ddPCR) to detect and quantify the frequency of the E119V- NA mutation. ...

Tick-Borne Encephalitis Virus Prevalence in Sheep, Wild Boar and Ticks in Belgium.

TBEV isolate, 1983 ticks collected in areas showing the highest TBEV seroprevalence in wild boars were tested by real-time qPCR. No TBEV- RNA-positive tick was detected. The results of this study suggest ...

A healthy lifestyle is positively associated with mental health and well-being and core markers in ageing

using qPCR. Generalised linear mixed models were used while adjusting for a priori chosen covariates. RESULTS: The average age (SD) of the study population was 49.9 (17.5) years, and 48.8% were men. ...

Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative.

available resources, putting in place a robust, validated, high-throughput, and decentralized qPCR molecular testing platform with embedded proficiency testing. We demonstrate how during an acute scarcity of ...

No molecular detection of tick-borne pathogens in the blood of patients with erythema migrans in Belgium

with fever after a recent tick bite were enrolled in the study. Three samples initially tested positive for N. mikurensis by quantitative PCR (qPCR), but the results could not be confirmed by other PCR ...

Higher buccal mtDNA content is associated with residential surrounding green in a panel study of primary school children

mitochondria DNA content (mtDNAc) in children. Methods In primary schoolchildren (COGNAC study), between 2012 and 2014, buccal mtDNAc was repeatedly (three times) assessed using qPCR. Surrounding low (<3m), ...

Orbivirus Screening from Imported Captive Oryx in the United Arab Emirates Stresses the Importance of Pre-Import and Transit Measures

about 4000 SHO s from two locations in the UAE were blood sampled to be tested by indirect enzyme-linked immunosorbent assay (ELISA) and real-time reverse transcriptase polymerase chain reaction (RT-qPCR ...

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