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Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system
five Escherichia coli pathotypes, based on a combination of 13 SYBR ® Green qPCR, has been developed, i.e., combinatory SYBR ® Green qPCR screening system for pathogenic E. coli (CoSYPS Path E. coli). It ...
Comparison of Droplet Digital PCR and qPCR for the Quantification of Shiga Toxin-Producing Escherichia coli in Bovine Feces.
PCR (qPCR), which has its specific limitations. Droplet digital PCR (ddPCR) has been proposed as a method to overcome many of the drawbacks of qPCR. This end-point amplification PCR is capable of ...