Search results - 122 results
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.
Publication Type: Peer reviewed scientific article Authors: Sylvia Broeders; Linda Garlant; Marie-Alice Fraiture; Els Vandermassen; Vanessa Suin; Jessica Vanhomwegen; Myrielle Dupont-Rouzeyrol; Dominique Rousset; Steven Van Gucht; Nancy Roosens Source: In ...
Combining a genotoxin-specific qPCR method with the ToxTracker to evaluate the genotoxic potential of high priority printed paper and board food contact material substances
Publication Type: Scientific poster, presentation or proceeding Authors: Melissa Van Bossuyt; G Hendriks; R Derr; TY Doktorova; Els Van Hoeck; T Vanhaecke; V Rogiers; Birgit Mertens Source: EEMGS meeting 2019, Rennes (France) (2019) URL: https://www.eemgs ...
Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study.
real-time polymerase chain reaction (RT-qPCR) directed against genomic regions conserved between different isolates. Genetic variation can however result in mismatches of primers and probes with their ...
Very Long Persistence of Botulinum Toxin B in a Patient’s Serum
immunocompromised adults) botulism. Case Report: A case of human botulism type B due to the consumption of an imported dried ham is reported. Laboratory results confirmed the diagnosis using the mouse bioassay and the qPCR ...
A novel genotoxin-specific qPCR array based on the metabolically competent human HepaRG™ cell line as a rapid and reliable tool for improved in vitro hazard assessment
Keersmaecker; Rogiers, Vera; Tatyana Y. Doktorova Source: Archives of Toxicology (2018) Keywords: genotoxicity HepaRG In vitro screening mutagenicity qPCR array Toxicogenomics Abstract: Although the value of the ...
Complete coding sequence of a novel picorna-like virus in a blackbird infected with Usutu virus.
consists of at least 9,228 nt and contains two open reading frames (ORF s) encoding the nonstructural polyprotein (2235 amino acids) and structural polyprotein (769 amino acids). Two TaqMan RT-qPCR assays ...
Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO.
region. This DNA walking is performed on transgenic elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this ...
Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes
Characterization detection DNA walking GMO identification qPCR Abstract: Background Recently, an integrated DNA walking strategy has been proposed to prove the presence of GMO via the characterisation of sequences ...
Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system
five Escherichia coli pathotypes, based on a combination of 13 SYBR ® Green qPCR, has been developed, i.e., combinatory SYBR ® Green qPCR screening system for pathogenic E. coli (CoSYPS Path E. coli). It ...
Pertussis diagnosis in Belgium: results of the National Reference Centre for Bordetella anno 2015.
Young adult Abstract: In 2015, the Belgian National Reference Centre for Bordetella analyzed 4110 respiratory samples by qPCR and 4877 serum samples by serology. Whereas about 50% of respiratory samples ...