Search results - 122 results

Evaluation of detection methods for non-O157 Shiga toxin-producing Escherichia coli from food.

in parallel in Buffered Peptone Water (BPW) and Brila broth. After enrichment, detection was performed using real-time PCR (qPCR), and isolation using two chromogenic agar media, CHROM agar™ STEC and ...

A molecular approach for the rapid, selective and sensitive detection of Exophiala jeanselmei in environmental samples: development and performance assessment of a real-time PCR assay

selectivity (100 %), PCR efficiency (95.5 %), dynamic range and repeatability of this qPCR assay were subsequently evaluated. The limit of detection for this qPCR assay was determined to be 1 copy of genomic ...

Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin.

approach. A TaqMan® qPCR method was developed and tested on isolated bacterial strains and on the feed additive directly. RESULTS: In this study, a very simple strategy based on the common BLAST tools that ...

The epidemiology of subclinical malaria infections in South-East Asia: findings from cross-sectional surveys in Thailand-Myanmar border areas, Cambodia, and Vietnam.

s), microscopy and a high volume ultra-sensitive real-time polymerase chain reaction (HVUS qPCR: limit of detection 22 parasites/mL). All villagers older than 6 months were invited to participate. ...

Current and new approaches in GMO detection: challenges and solutions

DNA, have been developed to implement these legislations. Due to its numerous advantages, the quantitative PCR (qPCR) is the method of choice for the enforcement laboratories in GMO routine analysis. ...

Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

Subsequently, the performance of this quantitative polymerase chain reaction (qPCR) method was assessed using specific criteria, including an evaluation of the selectivity, PCR efficiency, dynamic range, and ...

Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices.

with qPCR screening, usually used in GMO routine analysis, as well as being able to detect, characterize and identify a broad spectrum of GMO s in food/feed matrices, two bidirectional DNA walking ...

Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing

number and diversity of genetically modified (GM) crops has significantly increased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly complex and laborious. While several ...

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