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Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
enrichment. All protocols that have been proposed have been chosen to be as close as possible to the ones currently used in the reference laboratories so they can be adapted to be used in routine in the future. ...
A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products
for the possible scenarios to occur in routine setting. Both short and long read sequencing were used. This workflow paves the way for a strategy to detect and characterize unknown GMM s by enforcement ...
Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Combined Species Identification and Drug Sensitivity Testing in Mycobacteria.
for reference laboratories. Recent technical improvements in matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI- TOF MS) has started to facilitate routine mycobacterial ...
Strategies to detect unauthorized GMO in the food and feed chain (PhD thesis)
https://www.sciensano.be/en/biblio/strategies-detect-unauthorized-gmo-food-and-feed-chain-phd-thesis
enforcement laboratories is becoming complex due mainly to the increasing number and diversity of GMO. To cope with the problematic of EU unauthorized GMO, this PhD aims to improve and strengthen the existing ...
Strategies to detect unauthorized genetically modified organisms in food and feed chain
(EU). However, the implementation of these legislations by the enforcement laboratories is becoming complex due mainly to the increasing number and diversity of GMO. To cope with the problematic of EU ...
Current and new approaches in GMO detection: challenges and solutions
https://www.sciensano.be/en/biblio/current-and-new-approaches-gmo-detection-challenges-and-solutions
DNA, have been developed to implement these legislations. Due to its numerous advantages, the quantitative PCR (qPCR) is the method of choice for the enforcement laboratories in GMO routine analysis. ...
Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices.
developed DNA walking methods proposed here can be easily implemented in GMO routine analysis by the enforcement laboratories. In providing crucial information about the transgene flanking regions and/or the ...
New trait-specific qualitative SYBR®Green qPCR methods to expand the panel of GMO screening methods used in the CoSYPS
the methods to a second laboratory. Both methods allow a specific, sensitive and repeatable detection of the respective targets in food and feed samples and can be easily applied in a routine ...
A simple multi-residue method for the determination of pesticides in fruits and vegetables using a methanolic extraction and ultra-high-performance liquid chromatography-tandem mass spectrometry: optimization and extension of scope.
method for the analysis of large scope pesticides in routine laboratories. Health Topics: Food consumption and food safety Category: A1 FOS Classification: 3.03 Health sciences ...
An innovative integrated approach based on DNA walking to identify unauthorized GMOs
advantage to be easily implementable in GMO routine analysis by the enforcement laboratories. Health Topics: Food consumption and food safety Consommation et sécurité alimentaires Voedselconsumptie en ...