Search results - 298 results
Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study.
sensitivity and specificity. CONCLUSIONS: We provide a comprehensive overview of dengue virus RT-qPCR method performance that will aid appropriate method selection allowing to take specific measures that aim to ...
Subcutaneous Granulomatous Inflammation due to Basidiobolomycosis: Case Reports of 3 Patients in Buruli Ulcer Endemic Areas in Benin.
other diseases, such as soft tissue tumors, lymphoma, or Buruli ulcer in the preulcerative stage. Microbiological confirmation by PCR for and culture yield the most specific diagnosis, yet they are not ...
Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.
isolates and sensitivity towards sequencing coverage, which could be linked to the specific data analysis strategies used therein. To demonstrate the effect of particular data analysis steps, several ...
Performance of in silico models for mutagenicity prediction of food contact materials
(FCM) constituents were chosen as study substances since many of these lack experimental data, making them an interesting group for in silico screening. Accuracy, sensitivity, specificity, positive ...
Evaluation of mycobacteria-specific gamma interferon and antibody responses before and after a single intradermal skin test in cattle naturally exposed to M. avium subsp. paratuberculosis and experimentally infected with M. bovis.
infected with a Belgian field strain of M. bovis. Heparinized blood, serum and fecal samples were collected at regular time intervals for mycobacteria-specific IFN-γ release assays, antibody analysis and for ...
Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system
genes. uidA controls for E. coli presence and all the other genes are specific targets of E. coli pathotypes. For each gene, two primer pairs have been designed to guarantee a sufficient detection even in ...
Evaluation of the clinical performance of the HPV-Risk assay using the VALGENT-3 panel.
significantly higher specificity (relative specificity: 1.02; 95% CI: 1.01-1.04; p <0.001). For CIN2+, similar results were obtained with relative sensitivity of 0.98; 95%CI: 0.93-1.02 and relative specificity ...
Defining optimal triage strategies for hrHPV screen positive women- an evaluation of HPV 16/18 genotyping, cytology and p16/Ki-67 cyto-immunochemistry.
available. Sensitivity and specificity for the CIN2 + of these triage tests were evaluated. RESULTS: Absolute sensitivities of HPV 16/18 typing, LBC and p16/Ki-67 cyto-immunochemistry for CIN2 + detection were ...
Hepatitis E virus serology and PCR: does the methodology matter?
Viral Hepatitis E Hepatitis E virus Humans Immunoglobulin G Immunoglobulin M polymerase chain reaction Sensitivity and Specificity Serologic Tests Abstract: Hepatitis E virus (HEV) genotype 3 is an ...
Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.
Keywords: 5' Untranslated Regions Animals DNA Primers Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sensitivity ...