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Evaluation of three competitive ELISAs and a fluorescence polarisation assay for the diagnosis of bovine brucellosis.

adult cattle were tested. Individual test sensitivities (Se) and specificities (Sp) were estimated. Threshold assessment using the receiver operating characteristic method was also performed. The most ...

Distinction between persistent and transient infection in a bovine viral diarrhoea (BVD) control programme: appropriate interpretation of real-time RT-PCR and antigen-ELISA test results.

sampling. A highly significant difference in cycle threshold (Ct) values obtained by RT- PCR was observed between PI and TI animals. ROC analysis was performed to establish thresholds to confirm with high ...

Real-time PCR for simultaneous detection and genotyping of bovine viral diarrhea virus.

coefficients of variation of threshold cycle values in inter-runs were less than 2.2%. The correct classification of genotype I and II viruses was assessed by using reference strains and characterized field ...

Transmission of encephalomyocarditis virus (EMCV) among pigs experimentally quantified.

0.71 (95% CI =0.08-4.93). Combining the information from both experiments results in an estimate for R(0) of 1.24 (95% CI =0.39-4.35). Since R(0) has values around the threshold value of 1, the spread of ...

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