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Transforming Shiga toxin-producing surveillance through whole genome sequencing in food safety practices.
Nancy Roosens *; Sigrid C.J. De Keersmaecker * Source: Front Microbiol, Volume 14 (2023) Keywords: Food Safety implementation Shiga toxin-producing Escherichia coli Surveillance whole genome sequencing ...
Surveillance épidémiologique de l’Escherichia coli producteur de Shiga-Toxine (STEC), 2019-2020.
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Stéphanie Jacquinet; Florence Crombé; Denis Piérard Source: p.5 (0) Abstract: Depuis l’établissement du CNR en 2011, le nombre de cas annuel de STEC en Belgique est ...
Validation strategy of a bioinformatics whole genome sequencing workflow for Shiga toxin-producing Escherichia coli using a reference collection extensively characterized with conventional methods
performs complete characterization of Shiga toxin-producing Escherichia coli (STEC) isolates including antimicrobial resistance prediction, virulence gene detection, serotype prediction, plasmid replicon ...
Strain-Level Metagenomic Data Analysis of Enriched In Vitro and In Silico Spiked Food Samples: Paving the Way towards a Culture-Free Foodborne Outbreak Investigation Using STEC as a Case Study.
community obtained by in vitro spiking minced meat with a Shiga toxin-producing (STEC) isolate originating from a described outbreak. The more optimal tool Sigma was further evaluated using in silico ...
A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.
Shiga toxin-producing (STEC) as a case study. Different DNA extraction kits and enrichment procedures were investigated to obtain the most practical workflow. We demonstrated the feasibility of shotgun ...
The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country.
Vanneste; Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Foods, Volume 9, Issue 8 (2020) Keywords: Food Safety foodborne outbreak investigation Shiga toxin-producing Escherichia coli STEC Surveillance WGS ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates
C.J. De Keersmaecker Source: Scientific Reports, Volume 10, Issue 1 (2020) Keywords: DNA extraction kits outbreak investigation Shiga toxin producing Escherichia coli (STEC) Whole genome sequencing (WGS) ...
Global and regional source attribution of Shiga toxin-producing Escherichia coli infections using analysis of outbreak surveillance data
Publication Type: Peer reviewed scientific article Authors: Sara M. Pires; Shannon Majowicz; Alexander Gill; Brecht Devleesschauwer Source: Epidemiology and Infection, Volume 147 (2019) Health Topics: Pathogene E. coli Pathogenic E. coli E. coli pathogèn ...
Associating sporadic, foodborne illness caused by Shiga toxin-producing Escherichia coli with specific foods: a systematic review and meta-analysis of case-control studies
Publication Type: Peer reviewed scientific article Authors: Brecht Devleesschauwer; Sara Monteiro Pires; Ian Young; Alex Gill; Shannon E Majowicz; the study team Source: Epidemiology and Infection, Volume 147 (2019) Health Topics: Pathogenic E. coli Path ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates.
virulence and antimicrobial resistance genotyping) and phylogenetic relatedness (core genome multilocus sequence typing and single nucleotide polymorphism analysis). Shiga toxin-producing Escherichia coli ...