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Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants.
existing reverse transcriptase real-time PCR (RT-qPCR) assay, we developed a reverse transcriptase droplet digital PCR assay (RT-ddPCR) to detect and quantify the frequency of the E119V- NA mutation. ...
Redesign and Validation of a Real-Time RT-PCR to Improve Surveillance for Avian Influenza Viruses of the H9 Subtype.
Calogero; Monne, Isabella Source: Viruses, Volume 14, Issue 6 (2022) Keywords: Animals Humans Influenza A virus Influenza in Birds Poultry Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase ...
Orbivirus Screening from Imported Captive Oryx in the United Arab Emirates Stresses the Importance of Pre-Import and Transit Measures
about 4000 SHO s from two locations in the UAE were blood sampled to be tested by indirect enzyme-linked immunosorbent assay (ELISA) and real-time reverse transcriptase polymerase chain reaction (RT-qPCR) ...
Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study.
Abstract: BACKGROUND: Viral infection by dengue virus is a major public health problem in tropical countries. Early diagnosis and detection are increasingly based on quantitative reverse transcriptase ...
Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.
Keywords: 5' Untranslated Regions Animals DNA Primers Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sensitivity ...
Different measles outbreaks in Belgium, January to June 2016- a challenge for public health.
Personnel hospitals Humans Immunoglobulin M Infant Infectious Disease Transmission, Patient-to-Professional Male Measles Measles Vaccine middle aged public health Reverse Transcriptase Polymerase Chain ...
Reconstruction of the Schmallenberg virus epidemic in Belgium: Complementary use of disease surveillance approaches.
Surveillance Reverse Transcriptase Polymerase Chain Reaction Seroepidemiologic Studies Abstract: Schmallenberg virus (SBV) emerged across Europe in 2011 and Belgium was among the first countries affected. In ...
European interlaboratory comparison of Schmallenberg virus (SBV) real-time RT-PCR detection in experimental and field samples: The method of extraction is critical for SBV RNA detection in semen.
Transcriptase Polymerase Chain Reaction RNA, Viral semen Sensitivity and Specificity Abstract: Molecular methods for the detection of Schmallenberg virus (SBV) RNA were rapidly developed after the emergence of ...
Experimental Infection of Sheep at 45 and 60 Days of Gestation with Schmallenberg Virus Readily Led to Placental Colonization without Causing Congenital Malformations.
Transmission, Vertical Male Orthobunyavirus Placenta Pregnancy Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sheep Sheep Diseases Time Factors Vero Cells Abstract: BACKGROUND: Main impact of ...
Detection and Isolation of Swine Influenza A Virus in Spiked Oral Fluid and Samples from Individually Housed, Experimentally Infected Pigs: Potential Role of Porcine Oral Fluid in Active Influenza A Virus Surveillance in Swine.
(2015) Keywords: Animals Chick Embryo Influenza A Virus, H1N1 Subtype Influenza A Virus, H3N2 Subtype Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Saliva Swine ...