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Development of a reverse transcriptase digital droplet polymerase chain reaction‐based approach for SARS‐CoV‐2 variant surveillance in wastewater

Publication Type: Peer reviewed scientific article Authors: Laura Van Poelvoorde; Andrea Gobbo; S. Nauwelaerts; Bavo Verhaegen; Marie Lesenfants; Raphael Janssens; Veronik Hutse; Marie-Alice Fraiture; Sigrid C.J. De Keersmaecker; Philippe Herman; Koenraad ...

Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants.

existing reverse transcriptase real-time PCR (RT-qPCR) assay, we developed a reverse transcriptase droplet digital PCR assay (RT-ddPCR) to detect and quantify the frequency of the E119V- NA mutation. ...

Redesign and Validation of a Real-Time RT-PCR to Improve Surveillance for Avian Influenza Viruses of the H9 Subtype.

Calogero; Monne, Isabella Source: Viruses, Volume 14, Issue 6 (2022) Keywords: Animals Humans Influenza A virus Influenza in Birds Poultry Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase ...

Orbivirus Screening from Imported Captive Oryx in the United Arab Emirates Stresses the Importance of Pre-Import and Transit Measures

about 4000 SHO s from two locations in the UAE were blood sampled to be tested by indirect enzyme-linked immunosorbent assay (ELISA) and real-time reverse transcriptase polymerase chain reaction (RT-qPCR) ...

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