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BSFM 2023- L. monocytogenes in Plant-Based Foods: insights from of a recent foodborne outbreak linked to vegan alternative to cheese
following ISO 11290 – 1 and – 2. A human strain was collected at the NRC Listeria. All isolated strains underwent serotyping for further characterisation at the NRC Listeria, and Whole Genome Sequencing (WGS) ...
Introduction of WGS in food microbiology: advantages and challenges
Keywords: antimicrobial resistance (AMR) Food Safety foodborne outbreak investigation Monitoring SciCom Whole genome sequencing (WGS) Abstract: In October 2021 the Scientific Committee of the Federal Agency ...
Listeria surveillance in Belgium
evolution in molecular typing, the NRC completely switched to Whole-Genome-Sequencing (WGS) for cluster detection. Therefore, an in-house Galaxy-based bio-informatic pipeline was developed. For the strain ...
Strategies to detect unauthorized genetically modified organisms in food and feed chain
GMO in alimentary matrices using exclusively the NGS technology, through a whole genome sequencing (WGS) approach, was also investigated. As this last approach does not theoretically require any prior ...
Laboratory capability for molecular detection and confirmation of novel coronavirus in Europe, November 2012287
Transcriptase Polymerase Chain Reaction Rna Rna,Viral Sample Samples SB- IM SCREENING Sequence Analysis standards survey Sweden Target TESTING virology WHO whole genome world World Health World Health ...
DNase SISPA-next generation sequencing confirms Schmallenberg virus in Belgian field samples and identifies genetic variation in Europe.
4.27-4.89 log(10) RNA copies per µL did not allow identification using DN ase SISPA- NGS. This partial sequence information was compared to the whole genome sequence of SBV isolated from bovines in Germany, ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates.
C.J. De Keersmaecker Source: Sci Rep, Volume 10, Issue 1 (2020) Keywords: NGS pathogenic E. coli STEC WGS Abstract: Whole genome sequencing (WGS) has proven to be the ultimate tool for bacterial isolate ...
Dissecting the molecular evolution of fluoroquinolone-resistant Shigella sonnei.
antimicrobials, including the fluoroquinolones. Here we analyse nearly 400 S. sonnei whole genome sequences from both endemic and non-endemic regions to delineate the evolutionary history of the recently emergent ...
Global phylogenomics of multidrug-resistant Salmonella enterica serotype Kentucky ST198.
an emerging problem within this serotype. We used whole-genome sequencing (WGS) to investigate the phylogenetic structure and AMR content of 121 S.enterica serotype Kentucky sequence type 198 isolates ...
Integrative transnational analysis to dissect tuberculosis transmission events along the migratory route from Africa to Europe.
Mycobacterium tuberculosis isolates from these recently arrived migrants were analysed by Mycobacterial Interspersed Repetitive-Unit/Variable-Number of Tandem Repeat (MIRU- VNTR) and subsequent whole genome ...