Search results - 126 results

Strategy and Performance Evaluation of Low-Frequency Variant Calling for SARS-CoV-2 Using Targeted Deep Illumina Sequencing

The performance of detection and quantification of low-frequency variants using whole genome sequencing (WGS) of SARS-CoV-2 remains largely unknown. Here, we evaluated the detection and quantification ...

A robust, cost-effective and widely applicable whole-genome sequencing protocol for capripoxviruses

Publication Type: Peer reviewed scientific article Authors: Elisabeth Mathijs; Andy Haegeman; Kris De Clercq; Steven Van Borm; Frank Vandenbussche Source: Journal of Virological Methods (2022) Health Topics:  Animal health Santé animale Diergezondheid Ful ...

WGS- versus ORF5-Based Typing of PRRSV: A Belgian Case Study.

database is available. In this study, we performed whole-genome sequencing (WGS) on a collection of 124 PRRSV-1 positive serum samples that were collected over a 5-year period (2015-2019) in Belgium. Our ...

Retrospective evaluation of routine whole genome sequencing of Mycobacterium tuberculosis at the Belgian National Reference Center, 2019.

switched from conventional typing to prospective whole-genome sequencing (WGS) of all submitted complex (MTB) isolates. The ISO17025 validated procedure starts with semi-automated extraction and purification ...

Strategy to Develop and Evaluate a Multiplex RT-ddPCR in Response to SARS-CoV-2 Genomic Evolution.

continuously evolving worldwide. In the present study, we proposed a methodology based on an in silico and in vitro analysis. First, we used all information offered by available whole-genome sequencing data for ...

Application of a strain-level shotgun metagenomics approach on food samples: resolution of the source of a Salmonella food-borne outbreak

conventional analysis based on isolation, serotyping and MLVA (multilocus variable number tandem repeat analysis) comparison, followed by whole-genome sequencing, to confirm the source of the contamination over ...

Coverage of the national surveillance system for human Salmonella infections, Belgium, 2016-2020.

Food Poisoning Salmonella Infections whole genome sequencing Abstract: INTRODUCTION: The surveillance of human salmonellosis in Belgium is dependent on the referral of human Salmonella isolates to the ...

Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf

potentiated sulphonamides, streptomycin, tetracyclines and intermediate susceptibility for enrofloxacin. Whole genome sequencing confirmed identification as and the multidrug resistant character of the isolate. ...

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