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Validation of a Bioinformatics Workflow for Routine Analysis of Whole-Genome Sequencing Data and Related Challenges for Pathogen Typing in a European National Reference Center: as a Proof-of-Concept.
Keersmaecker; Nancy Roosens; Kevin Vanneste Source: Front Microbiol, Volume 10 (2019) Keywords: national reference center Neisseria meningitidis public health VALIDATION whole-genome sequencing Abstract: Despite ...
Bigger and Better? Representativeness of the Influenza A Surveillance Using One Consolidated Clinical Microbiology Laboratory Data Set as Compared to the Belgian Sentinel Network of Laboratories.
subject to local regional variations. A subset of the Influenza samples had their whole genome sequenced so that the observed epidemiological trends could be correlated to molecular observations from the ...
Antibiotic resistance prediction for Mycobacterium tuberculosis from genome sequence data with Mykrobe.
Organization priority. We previously developed a software tool,, which provided offline species identification and drug resistance predictions for from whole genome sequencing (WGS) data. Performance was ...
Detailed Evaluation of Data Analysis Tools for Subtyping of Bacterial Isolates Based on Whole Genome Sequencing: as a Proof of Concept.
Volume 10 (2019) Abstract: Whole genome sequencing is increasingly recognized as the most informative approach for characterization of bacterial isolates. Success of the routine use of this technology in ...
Comparative Analysis of Whole-Genome Sequence of African Swine Fever Virus Belgium 2018/1.
25, Issue 6 (2019) Abstract: We analyzed the whole-genome sequence of African swine fever virus Belgium 2018/1. The strain fits into the European genotype II (>99.98% identity). The high-coverage ...
Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java.
biochemical tests. Therefore, a quadruplex real-time PCR method, based on the allelic discrimination of molecular markers selected from the scientific literature and from whole genome sequencing data produced ...
Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.
respectively. All discrepancies were analyzed with whole-genome sequencing and shown to be related to causative mutations (stop codons, indels, and promoter mutations) in glycosyltransferase genes. This ...
Occurrence and identification of spore-forming bacteria in skim-milk powders
manufacturing environments. Using whole genome sequencing, 16 of 23 isolates identified as B. licheniformis by 16S sequencing were confirmed as B. licheniformis, four were identified as Bacillus ...
Seasonal Genetic Drift of Human Influenza A Virus Quasispecies Revealed by Deep Sequencing
determine the whole genome genetic heterogeneity of the three categories of influenza A viruses that circulated in humans between 2007 and 2012 in France, directly from clinical respiratory specimens. ...
Prediction of Susceptibility to First-Line Tuberculosis Drugs by DNA Sequencing
susceptibility to first-line antituberculosis drugs has not been clear. METHODS: We obtained whole-genome sequences and associated phenotypes of resistance or susceptibility to the first-line antituberculosis ...