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Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates.

Keersmaecker, S C J * Source: Sci Rep, Volume 10, Issue 1 (2020) Keywords: NGS pathogenic E. coli STEC WGS Abstract: Whole genome sequencing (WGS) has proven to be the ultimate tool for bacterial isolate ...

High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei.

from the whole genome sequences of 45 B. mallei isolates identified 3 lineages for this species. In this study, we designed a high-resolution melting (HRM) method for the screening of 15 phylogenetically ...

The AMR-ARRAY: A modular bead array detecting β-lactam, (fluoro) quinolone, colistin, aminoglycoside and macrolide resistance determinants in Gram-negative bacteria.

phenotypically expressed resistance for 94.7% (856/904) of the assessed resistances. When compared to resistance profiles inferred from whole genome sequencing results, the AMR- ARRAY showed a selectivity and ...

Monitoring of influenza: Whole-Genome Sequencing to provide insights in the disease severity

Publication Type: Scientific poster, presentation or proceeding Authors: Laura Van Poelvoorde; Sigrid De Keersmaecker; Kevin Vanneste; Steven Van Gucht; Isabelle Thomas; Xavier Saelens; Cyril Barbezange; Nancy Roosens Source: The 6th International Influen ...

Status and potential of bacterial genomics for public health practice: a scoping review

of whole-genome sequencing (WGS) data for (1) source tracing, (2) early outbreak detection, (3) unraveling transmission dynamics, (4) monitoring drug resistance, (5) detecting cross-border transmission ...

Integrative transnational analysis to dissect tuberculosis transmission events along the migratory route from Africa to Europe.

Mycobacterium tuberculosis isolates from these recently arrived migrants were analysed by Mycobacterial Interspersed Repetitive-Unit/Variable-Number of Tandem Repeat (MIRU- VNTR) and subsequent whole genome ...

Whole-genome-based phylogenomic analysis of the Belgian 2016-2017 influenza A(H3N2) outbreak season allows improved surveillance.

Human Phylogeny Public Health Surveillance whole genome sequencing Abstract: Seasonal influenza epidemics are associated with high mortality and morbidity in the human population. Influenza surveillance ...

A general approach to identify low-frequency variants within influenza samples collected during routine surveillance.

concept for the added value of LFV for routine influenza monitoring, potential associations between patient data and whole genome sequencing data were investigated. A significant association was found ...

Development and evaluation of a core genome multilocus sequence typing scheme for Paenibacillus larvae, the deadly American foulbrood pathogen of honeybees.

Genome, Bacterial Multilocus Sequence Typing Paenibacillus larvae whole genome sequencing Abstract: Paenibacillus larvae is the causative agent of the fatal American foulbrood disease in honeybees (Apis ...

Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing.

Sci Rep, Volume 10, Issue 1 (2020) Keywords: Drug Resistance, Bacterial Food Microbiology Genetic Vectors Microorganisms, Genetically-Modified Peptide Hydrolases whole genome sequencing Abstract: ...

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