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Staphylococcal enterotoxin gene cluster: prediction of enterotoxin (SEG and SEI) production and of the source of food poisoning based on Saβ typing.

Graber, H U Source: Appl Environ Microbiol (2020) Keywords: S. aureus SEG SEI vSaβ type whole genomic sequencing Abstract: Currently only five (SEA- SEE) out of 27 known staphylococcal enterotoxins can be ...

Genomic epidemiology of emerging ESBL-producing Kentucky in Europe.

urinary-tract infections. Bayesian time-scaled phylogeny on whole genome sequences of isolates from these cases and supplementary isolates from public sequence databases was used to infer the origin and spread of ...

Deep amplicon sequencing for culture-free prediction of susceptibility or resistance to 13 anti-tuberculous drugs.

J (2020) Abstract: Conventional molecular tests for detecting complex (MTBC) drug resistance on clinical samples cover a limited set of mutations. Whole genome sequencing (WGS) typically requires culture. ...

Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination.

view of AMR gene localization, i.e., chromosomal or on a plasmid. Whole-genome and plasmid DNA extraction methods were compared, as were assemblies consisting of short reads (Illumina MiSeq), long reads ...

A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.

De Keersmaecker, Sigrid Source: Microorganisms, Volume 8, Issue 8 (2020) Keywords: food surveillance Metagenomics outbreak SNP analysis STEC whole genome Abstract: The management of a foodborne ...

Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.

Polymorphism, Single Nucleotide Real-Time Polymerase Chain Reaction RNA Replicase RNA, Viral Sensitivity and Specificity whole genome sequencing Abstract: The current CO ronaVIrus Disease 2019 (COVID-19) pandemic ...

The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country.

WGS whole genome sequencing Abstract: Gradually, conventional methods for foodborne pathogen typing are replaced by whole genome sequencing (WGS). Despite studies describing the overall benefits, ...

Use of a Whole Genome Sequencing-based approach for Mycobacterium tuberculosis surveillance in Europe in 2017-2019: an ECDC pilot study.

Dick van Soolingen; Marieke J van der Werf; Daniela Maria Cirillo; ECDC molecular surveillance project participants Source: Eur Respir J (2020) Abstract: Whole genome sequencing (WGS) can be used for ...

Food Chemistry, Function and AnalysisDNA Techniques to Verify Food Authenticity CHAPTER 8. GMO Detection and Identification Using Next-generation Sequencing

approaches can be applied. The whole-genome sequencing approach, on the one hand, requires no prior knowledge, and is suitable for the characterization of samples containing an isolated single GMO. The ...

Phylogeography of Human and Animal Strains: Genetic Fingerprinting of Q Fever in Belgium.

reservoirs. To determine the relationship between animal and human strains, Multiple Locus Variable-number Tandem Repeat Analysis (MLVA) (n=146), Single-Nucleotide Polymorphism (SNP) (n=92) and Whole Genome ...

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