A non-invasive intranasal inoculation technique using isoflurane anesthesia to infect the brain of mice with rabies virus.

Last updated on 22-8-2019 by Anonymous (non vérifié)

Peer reviewed scientific article



Methods for intranasal inoculation of viruses are often described poorly and the effects of variations in the technique on the outcome are unknown. Standardization of protocols is key to compare studies and minimize animal use. The clinical and virological outcome of infection with rabies virus (genotypes 1 and 5) upon administration of different inoculum volumes (25, 50 and 100μl) and different anesthetic regimens were examined. Administration of 25μl of virus as a drop on both nostrils under brief superficial isoflurane anesthesia (92μl/dm(3), recovery after 85 ± 1 0s) was the most effect…

Associated health topics:

QR code

QR code for this page URL