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Detection of single cells of Cryptococcus neoformans in clinical samples by solid-phase cytometry36716

Last updated on 22-8-2019 by Anonymous (non vérifié)

Public Access

Published

Peer reviewed scientific article

SCIENSANO

Auteurs

Bauters,T.G.; Swinne,D.; Stove,V.; H. Nelis

Mots-clés

  1. a
  2. analysi
  3. analysis
  4. article
  5. Belgique
  6. Cell
  7. cells
  8. cerebrospinal fluid
  9. Clinical
  10. Clinical samples
  11. Cryptococcosis
  12. Cryptococcus
  13. cytology
  14. detection
  15. Diagnosis
  16. EVALUATION
  17. Flow Cytometry
  18. Fluorescence
  19. Fluorescent Antibody Technique
  20. growth & development
  21. Humans
  22. im
  23. IS
  24. isolation & purification
  25. journal
  26. labeling
  27. Laboratories
  28. Meningitis,Cryptococcal
  29. method
  30. methods
  31. microbiology
  32. ON
  33. Imprimer
  34. Research
  35. Research Support
  36. Sample
  37. Samples
  38. SB - IM
  39. serum
  40. study
  41. Time Factors
  42. Universities
  43. university
  44. viability

Résumé:

A method based on solid-phase cytometry for the detection and enumeration of single cells of Cryptococcus neoformans in serum and cerebrospinal fluid is described. Both viable and nonviable cells are detected by using fluorescence viability labeling and immunofluorescence. This 30-min procedure has a detection limit of 3 to 6 cells per ml
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