Evaluation of viability-qPCR detection system on viable and dead Salmonella serovar Enteritidis.

Last updated on 22-8-2019 by Anonymous (non vérifié)

Peer reviewed scientific article

SCIENSANO

Résumé:

The propidium monoazide (PMA) coupled with PCR (viability PCR) is used in foodborne pathogen detection in order to detect only viable bacteria. Originally presented to fully remove the signal of dead bacteria, the limits of the viability PCR rapidly came out in the literature. In this study, the use of PMA in a viability-qPCR (v-qPCR) was assessed on viable and dead cells of Salmonella enterica subsp. enterica serovar Enteritidis. The PMA treatment protocol was modified (dark incubation duration, concentration of PMA) to evaluate if a complete negative signal of dead Salmonella was possible…

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