TY - JOUR T1 - Determination of bixin and norbixin in meat using liquid chromatography and photodiode array detection JF - Food Addit.Contam Part A Chem.Anal.Control Expo.Risk Assess. Y1 - 2009 A1 - Noppe,H. A1 - S. Abuin Martinez A1 - Verheyden,K. A1 - Joris Van Loco A1 - Companyo Beltran,R. A1 - H.F. De Brabander KW - an KW - analysis KW - Calibration KW - chromatography KW - Commission Decision 2002/657/EC KW - Decision KW - detection KW - Development KW - dye KW - HPLC KW - Ion KW - IS KW - liquid chromatography KW - Mass KW - Mass Spectrometry KW - Meat KW - method KW - performance KW - Quantification KW - RANGE AB - The development of an analytical method that enables routine analysis of annatto dye, specifically bixin and norbixin, in meat tissue is described. Liquid-solid extraction was carried out using acetonitrile. Analysis was by HPLC with photodiode array detection using two fixed wavelengths (458 and 486 nm). The possibilities of ion trap mass spectrometry (MS) were also assessed. Method performance characteristics, according to Commission Decision 2002/657/EC, were determined, with recoveries between 99 and 102% and calibration curves being linear in the 0.5-10 mg kg(-1) range. The limit of quantification was 0.5 mg kg(-1). VL - 26 CP - 1 U1 - 2089 M3 - http://dx-doi.org/10.1080/02652030802322564 ER -