TY - JOUR T1 - Use of PFGE to characterize clonal relationships among Belgian clinical isolates of Listeria monocytogenes36739 JF - J.Med.Microbiol. Y1 - 2004 A1 - Yde,M. A1 - Genicot,A. KW - 2001 KW - a KW - an KW - application KW - Arsenic KW - article KW - Bacterial Typing Techniques KW - Belgian KW - Belgium KW - Brussels KW - Cadmium KW - classification KW - Clinical KW - data KW - Deoxyribonucleases,Type II Site-Specific KW - Electrophoresis KW - Electrophoresis,Gel,Pulsed-Field KW - EPIDEMIOLOGICAL KW - epidemiology KW - EVALUATION KW - general KW - genetics KW - health KW - Human KW - Humans KW - identify KW - im KW - Institute KW - IS KW - isolation & purification KW - journal KW - Listeria KW - Listeria monocytogenes KW - Listeriosis KW - metabolism KW - methods KW - Microbial Sensitivity Tests KW - microbiology KW - occurrence KW - ON KW - pharmacology KW - Print KW - profile KW - Profiles KW - public KW - public health KW - Public-health KW - report KW - resistance KW - restriction KW - SB - IM KW - Serotyping KW - strain KW - study KW - Type KW - use AB - The Belgian Listeria Reference Centre receives between 30 and 50 human clinical strains of Listeria monocytogenes per year. In general, epidemiological data are absent or incomplete, preventing recognition of episodes of listeriosis. However, data on a clonal relationship between strains can indirectly give an idea of the occurrence of episodes. Human isolates of L. monocytogenes from 2001 were serotyped, their arsenic-cadmium resistance profiles were determined, and they were pulsotyped with the application of pulsed-field gel electrophoresis using AscI and ApaI restriction endonucleases. On five occasions, two or more strains presented the same serovar, metal-resistance profile and pulsovar, suggesting a clonal relationship. This is the first report to identify accurately potential listeriosis episodes occurring in Belgium VL - 53 CP - Pt 5 U1 - 38477 M3 - http://dx.doi.org/ ER -