TY - JOUR T1 - Comparison of two real-time RT-PCR assays for differentiation of C-strain vaccinated from classical swine fever infected pigs and wild boars. JF - Res Vet Sci Y1 - 2014 A1 - Widén, F A1 - Everett, H A1 - Blome, S A1 - J Fernandez Pinero A1 - Uttenthal, A A1 - Cortey, M A1 - von Rosen, T A1 - Marylène Tignon A1 - Liu, L KW - Animals KW - Antibodies, Viral KW - Classical Swine Fever KW - Classical swine fever virus KW - Diagnosis, Differential KW - Diagnostic Tests, Routine KW - DNA, Viral KW - European Union KW - Genotype KW - Real-Time Polymerase Chain Reaction KW - Reverse Transcriptase Polymerase Chain Reaction KW - Sensitivity and Specificity KW - Sus scrofa KW - Swine KW - Vaccination KW - Viral Vaccines AB -

Classical swine fever is one of the most important infectious diseases for the pig industry worldwide due to its economic impact. Vaccination is an effective means to control disease, however within the EU its regular use is banned owing to the inability to differentiate infected and vaccinated animals, the so called DIVA principle. This inability complicates monitoring of disease and stops international trade thereby limiting use of the vaccine in many regions. The C-strain vaccine is safe to use and gives good protection. It is licensed for emergency vaccination in the EU in event of an outbreak. Two genetic assays that can distinguish between wild type virus and C-strain vaccines have recently been developed. Here the results from a comparison of these two real-time RT-PCR assays in an interlaboratory exercise are presented. Both assays showed similar performance.

VL - 97 CP - 2 U1 - http://www.ncbi.nlm.nih.gov/pubmed/25017044?dopt=Abstract M3 - 10.1016/j.rvsc.2014.06.010 ER -