%0 Journal Article %J J.Hosp.Infect. %D 1996 %T Changes in major populations of methicillin-resistant Staphylococcus aureus in Belgium36669 %A C. Wildemauwe %A Godard,C. %A R. Vanhoof %A Bossuyt,E.V. %A Hannecart-Pokorni,E. %K 0 %K a %K Agent %K Agents %K ALL %K Aminoglycosides %K Anti-Bacterial Agents %K article %K Bacteriophage Typing %K Belgian %K Belgium %K Change %K Changes %K classification %K Combination %K Cross Infection %K differences %K Dna %K DNA Fingerprinting %K DNA,Bacterial %K drug effects %K Drug Resistance,Microbial %K Electrophoresis,Gel,Pulsed-Field %K Enzymes %K enzymology %K epidemic %K epidemiology %K Group %K hospital %K hospitals %K Humans %K im %K IS %K journal %K Methicillin Resistance %K Methicillin-Resistant Staphylococcus aureus %K method %K microbiology %K MRSA %K period %K Phage type %K pharmacology %K polymerase chain reaction %K POPULATION %K Populations %K prevalence %K Print %K SB - IM %K Staphylococcal Infections %K staphylococcus %K Staphylococcus aureus %X A total of 102 epidemic methicillin-resistant Staphylococcus aureus (MRSA) isolates collected in 13 Belgian hospitals during two periods (1981-1985 and 1991-1992) were tested for phage-type, for the presence of aminoglycoside-modifying enzymes (AME), and examined by arbitrarily primed polymerase chain reaction (AP-PCR). All isolates, but five, belonged to a few distinct phage-types of group III. Most isolates expressed a combination of AAC(6')-APH(2") with APH(3')III, and ANT(4',4") or both. Both phage-typing and AME suggested a change in the MRSA population between the two periods but the AP-PCR method revealed only slight differences %B J.Hosp.Infect. %V 34 %P 197 - 203 %8 0/11/1996 %G eng %N 3 %1 38470 %& 197