%0 Journal Article %J Vaccine %D 2009 %T Some guidelines for determining foot-and-mouth disease vaccine strain matching by serology. %A Mattion, Nora %A Goris, Nesya %A Willems, Tom %A Robiolo, Blanca %A Maradei, Eduardo %A Beascoechea, Claudia Perez %A Perez, Alejandro %A Smitsaart, Eliana %A Fondevila, Norberto %A Palma, Eduardo %A Kris De Clercq %A La Torre, José %K Animals %K Antibodies, Viral %K Argentina %K Cattle %K Cross Reactions %K Disease Outbreaks %K Enzyme-Linked Immunosorbent Assay %K Foot-and-Mouth Disease %K Foot-and-Mouth Disease Virus %K Immunoassay %K Neutralization Tests %K Viral Vaccines %X

The selection of matching strains for use in outbreaks of foot-and-mouth disease (FMD) virus can be assessed in vivo or by serological r-value determination. Sera from animals involved in vaccine potency and cross-protection trials performed using the "Protection against Podal Generalization" (PPG) test for two serotype A strains were collected and analyzed by the virus neutralization test (VNT) and liquid-phase ELISA (lpELISA) in three laboratories. The average VNT r-values for medium and high serum titer classes from the A(24) Cruzeiro vaccinated animals were in line with the A/Arg/01 heterologous PPG outcome for all testing laboratories, suggesting that the vaccine strain A(24) Cruzeiro is unlikely to protect against the field isolate A/Arg/01. The corresponding lpELISA r-values were slightly higher and indicate a closer relationship between both strains. Pooling of serum samples significantly reduced the inter-animal and inter-trial variation. The results suggest that a suitable reference serum for vaccine matching r-value experiments might be a pool or a medium to high VNT or lpELISA titer serum. Furthermore, the VNT seems to produce the most reproducible inter-laboratory results. More work is, however, needed in order to substantiate these claims.

%B Vaccine %V 27 %P 741-7 %8 2009 Jan 29 %G eng %N 5 %1 http://www.ncbi.nlm.nih.gov/pubmed/19041355?dopt=Abstract %R 10.1016/j.vaccine.2008.11.026