%0 Journal Article %J Korean J Parasitol %D 2002 %T Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs. %A De Graaf, Dirk C %A De Coninck, Hans %A Petry, Franz %A Eeckhout, Ilka B %A Johan Peeters %K Amino Acid Sequence %K Animals %K Antibodies, Protozoan %K Antigens, Protozoan %K Base Sequence %K Cattle %K Cryptosporidium parvum %K Molecular Sequence Data %K Protozoan Proteins %K Rabbits %K Recombinant Proteins %K Zinc Fingers %X

A Cryptosporidium parvum sporozoite and oocyst lambda gt11 cDNA library was screened with a hyperimmune rabbit serum that was developed against insoluble fragments of ultrasonicated oocysts. A clone named Cp22.4.1 encoding a protein of 231 amino acids with 4 zinc-finger domains characterized by a Cys-X2-Cys-X4-His-X4-Cys motif was isolated and characterized. There was a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD-TOPO-TA expression vector permitted to evaluate the antigenicity of the recombinant His-tagged antigen. This antigen was recognized by 2 out of 5 sera from Cryptosporidium immune calves and not by sera from parasite naive animals.

%B Korean J Parasitol %V 40 %P 59-64 %8 2002 Mar %G eng %N 1 %1 http://www.ncbi.nlm.nih.gov/pubmed/11949214?dopt=Abstract