%0 Journal Article %J N Biotechnol %D 2014 %T Engineering nucleases for gene targeting: safety and regulatory considerations. %A Katia Pauwels %A Podevin, Nancy %A Didier Breyer %A Carroll, Dana %A Philippe Herman %K Deoxyribonucleases %K Gene Targeting %K Genetic Therapy %K Humans %K SAFETY %X

Nuclease-based gene targeting (NBGT) represents a significant breakthrough in targeted genome editing since it is applicable from single-celled protozoa to human, including several species of economic importance. Along with the fast progress in NBGT and the increasing availability of customized nucleases, more data are available about off-target effects associated with the use of this approach. We discuss how NBGT may offer a new perspective for genetic modification, we address some aspects crucial for a safety improvement of the corresponding techniques and we also briefly relate the use of NBGT applications and products to the regulatory oversight.

%B N Biotechnol %V 31 %P 18-27 %8 2014 Jan 25 %G eng %N 1 %1 http://www.ncbi.nlm.nih.gov/pubmed/23851284?dopt=Abstract %& 18 %R 10.1016/j.nbt.2013.07.001