%0 Journal Article %J Food Addit Contam Part A Chem Anal Control Expo Risk Assess %D 2011 %T Cross-reactivity of some commercially available deoxynivalenol (DON) and zearalenone (ZEN) immunoaffinity columns to DON- and ZEN-conjugated forms and metabolites. %A Veršilovskis, A %A Bart Huybrechts %A Emmanuel Tangni %A Pussemier, L %A S. De Saeger %A A. Callebaut %K Animals %K Antibody Specificity %K Cattle %K Chromatography, Affinity %K Chromatography, High Pressure Liquid %K Immunoassay %K serum %K Tandem Mass Spectrometry %K Trichothecenes %K Zearalenone %X

Seven commercially available deoxynivalenol (DON) and zearalenone (ZEN) immunoaffinity columns (IACs) were tested for cross-reactivity to conjugated forms (3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, DON-3-glucoside, DON-3-glucuronide, ZEN-glucosides, ZEN-glucuronide) and metabolites (de-epoxydeoxynivalenol, α-zearalenol, β-zearalenol) and nivalenol (NIV), using a semi-quantitative multi-mycotoxin ultra-performance liquid chromatography-tandem mass spectrometry method. The DON IACs showed cross-reactivity for nearly all DON derivatives tested. The ZEN IACs showed limited cross-reactivity to some of the ZEN derivatives. The IACs were evaluated for their potential use as sample clean-up for mycotoxins in serum.

%B Food Addit Contam Part A Chem Anal Control Expo Risk Assess %V 28 %P 1687-93 %8 2011 Dec %G eng %N 12 %1 http://www.ncbi.nlm.nih.gov/pubmed/21801078?dopt=Abstract %R 10.1080/19440049.2011.603364