%0 Journal Article %J J Virol Methods %D 2020 %T An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies. %A Andy Haegeman %A Ilse De Leeuw %A Laurent Mostin %A Willem Van Campe %A Laetitia Aerts %A Maria Vastag %A Kris De Clercq %K antibodies %K IPMA %K LSDV. %X

During this study a new Immunoperoxidase Monolayer Assay (IPMA) was developed for the detection of antibodies against lumpy skin disease virus (LSDV) in an easy and low tech setting. Using two dilutions (1:50 and 1:300) in a duplicate format, the test was shown to be highly sensitive, specific and repeatable. In comparison to the VNT and a commercial ELISA, the LSDV-IPMA was able to detect the LSDV antibodies earlier in infected, vaccinated and vaccinated/infected animals. The assay is very flexible as it can be easily adapted for the detection of sheeppox or goatpox antibodies and it can be scaled-up to handle medium size sample sets by preparing the IPMA plates in advance. These plates are safe and can be handled in low biosafety level labs.

%B J Virol Methods %V 277 %8 2020 03 %G eng %R 10.1016/j.jviromet.2019.113800