<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>13</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">An Van den Bossche</style></author><author><style face="normal" font="default" size="100%">Pieter-Jan Ceyssens</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">WIV-ISP's experiences in the use of MALDI-TOF for the identification of Mycobacteria and their drug resistance profiles</style></title></titles><keywords><keyword><style  face="normal" font="default" size="100%">drug susceptibility testing</style></keyword><keyword><style  face="normal" font="default" size="100%">identification</style></keyword><keyword><style  face="normal" font="default" size="100%">MALDI-TOF</style></keyword><keyword><style  face="normal" font="default" size="100%">Mycobacterium tuberculosis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2016</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">FARES/VRGT </style></publisher><pub-location><style face="normal" font="default" size="100%">FARES/VRGT Brussels, Belgium</style></pub-location><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;An Van den Bossche presented WIV-ISP&amp;#39;s experiences in the use of Br&amp;uuml;ker&amp;rsquo;s Biotyper MALDI-TOF for the identification of Mycobacteria and their drug resistance profiles. By using an optimized extraction protocol, they successfully identified 88.14 % of 194 Mycobacterial strains at a species-level and 3.09 % at genus-level, using the Br&amp;uuml;ker Database v3.0. For organisms of the Mycobacterium tuberculosis complex, identifications were 100% correct, while the concordance with standard methods was a bit lower for Nontuberculous Mycobacteria, 88.03%. Five organisms were misidentified. A novel method of drug resistance testing was also presented, which uses semi-quantitative measurement of bacterial biomass by creating MALDI-TOF spectra in the presence of an internal standard.&lt;/p&gt;
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