<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Koen De Cremer</style></author><author><style face="normal" font="default" size="100%">Ilse Van Overmeire</style></author><author><style face="normal" font="default" size="100%">Joris Van Loco</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">On-line solid-phase extraction with ultra performance liquid chromatography and tandem mass spectrometry for the detection of nicotine, cotinine and trans-3'-hydroxycotinine in urine to strengthen human biomonitoring and smoking cessation studies.</style></title><secondary-title><style face="normal" font="default" size="100%">J Pharm Biomed Anal</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J Pharm Biomed Anal</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Chromatography, Liquid</style></keyword><keyword><style  face="normal" font="default" size="100%">cotinine</style></keyword><keyword><style  face="normal" font="default" size="100%">environmental exposure</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Nicotine</style></keyword><keyword><style  face="normal" font="default" size="100%">Reproducibility of Results</style></keyword><keyword><style  face="normal" font="default" size="100%">Sensitivity and Specificity</style></keyword><keyword><style  face="normal" font="default" size="100%">SMOKING</style></keyword><keyword><style  face="normal" font="default" size="100%">Smoking cessation</style></keyword><keyword><style  face="normal" font="default" size="100%">Solid phase extraction</style></keyword><keyword><style  face="normal" font="default" size="100%">Tandem Mass Spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Tobacco Smoke Pollution</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013 Mar 25</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">76</style></volume><pages><style face="normal" font="default" size="100%">126-33</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;At the time of writing, this work appears to be the first published report on the coupling of on-line solid phase extraction (SPE) with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the simultaneous detection of nicotine, cotinine and trans-3'-hydroxycotinine in human urine. The advantages of both on-line SPE (speed, automation, less labor intensive) coupled with UPLC-MS/MS (speed, sensitivity) offer a viable option for efficient and economical biomonitoring studies for the assessment of active and passive exposure to tobacco smoke and clinical studies focusing on smoke cessation techniques. In a first approach, a 1:100 dilution of the urine was applied to screen for both passive and active exposure. Intra- and inter-batch reproducibility of this fast method (10min) was assessed for three concentration levels and were found to be less than 8% for each analyte while the accuracy was between 89 and 113%. To further improve the sensitivity of this approach when focusing only on passive smokers, the performance of a 1:10 dilution with the on-line SPE UPLC-MS/MS system was also tested. Results indeed show better sensitivity (LOQ's 1.0, 1.0 and 5.0μg/l for respectively cotinine, nicotine and trans-3'-hydroxycotinine) and good analytical performance for all other analytical parameters on the low levels tested here. Both methods were applied to measure the concentration of nicotine, cotinine and trans-3'-hydroxycotinine in the urine of 53 volunteers (smokers and non-smokers) recruited via an internal call at the Scientific Institute of Public Health. For non-smokers and smokers, cotinine levels e.g. were respectively between 1.0-470μg/l and 97-2381μg/l. Both these on-line SPE UPLC-MS/MS methods showed their potential for dedicated future large biomonitoring projects as they made it possible to analyze large series of samples in a fast, sensitive, robust and cost-efficient manner.&lt;/p&gt;</style></abstract><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/23313774?dopt=Abstract</style></custom1></record></records></xml>