<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fernando Alcaide</style></author><author><style face="normal" font="default" size="100%">J Amlerová</style></author><author><style face="normal" font="default" size="100%">Germán Bou</style></author><author><style face="normal" font="default" size="100%">Pieter-Jan Ceyssens</style></author><author><style face="normal" font="default" size="100%">Pere Coll</style></author><author><style face="normal" font="default" size="100%">Dan Corcoran</style></author><author><style face="normal" font="default" size="100%">Marie-Sarah Fangous</style></author><author><style face="normal" font="default" size="100%">Iván González-Álvarez</style></author><author><style face="normal" font="default" size="100%">Rebecca Gorton</style></author><author><style face="normal" font="default" size="100%">Gilbert Greub</style></author><author><style face="normal" font="default" size="100%">Geneviève Hery-Arnaud</style></author><author><style face="normal" font="default" size="100%">Jaroslav Hrábak</style></author><author><style face="normal" font="default" size="100%">André Ingebretsen</style></author><author><style face="normal" font="default" size="100%">Brigid Lucey</style></author><author><style face="normal" font="default" size="100%">Ivana Marekoviċ</style></author><author><style face="normal" font="default" size="100%">Concepción Mediavilla-Gradolph</style></author><author><style face="normal" font="default" size="100%">James O'Connor</style></author><author><style face="normal" font="default" size="100%">Jim O'Mahony</style></author><author><style face="normal" font="default" size="100%">Onya Opota</style></author><author><style face="normal" font="default" size="100%">Brendan O'Reilly</style></author><author><style face="normal" font="default" size="100%">Dorothea Orth-Höller</style></author><author><style face="normal" font="default" size="100%">Marina Oviaño</style></author><author><style face="normal" font="default" size="100%">Juan José Palacios</style></author><author><style face="normal" font="default" size="100%">Begoña Palop</style></author><author><style face="normal" font="default" size="100%">Arthur Pranada</style></author><author><style face="normal" font="default" size="100%">Lidia Quiroga</style></author><author><style face="normal" font="default" size="100%">David Rodríguez-Temporal</style></author><author><style face="normal" font="default" size="100%">María Jesús Ruiz-Serrano</style></author><author><style face="normal" font="default" size="100%">Griselda Tudó</style></author><author><style face="normal" font="default" size="100%">An Van den Bossche</style></author><author><style face="normal" font="default" size="100%">Jakko van Ingen</style></author><author><style face="normal" font="default" size="100%">Belén Rodriguez-Sanchez</style></author></authors><translated-authors><author><style face="normal" font="default" size="100%">European Study Group on Genomics and Molecular Diagnosis (ESGMD)</style></author></translated-authors></contributors><titles><title><style face="normal" font="default" size="100%">How To: Identify Non-Tuberculous Mycobacterium Species By Using Maldi-Tof Mass Spectrometry.</style></title><secondary-title><style face="normal" font="default" size="100%">Clin Microbiol Infect</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">MALDI-TOF MS</style></keyword><keyword><style  face="normal" font="default" size="100%">Non-Tuberculoous Mycobacteria</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2017 Nov 21</style></date></pub-dates></dates><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;b&gt;BACKGROUND: &lt;/b&gt;The implementation of MALDI-TOF MS for microorganism identification has changed the routine of the microbiology laboratories as we knew it. Most microorganisms can now be reliably identified within minutes using this inexpensive, user-friendly methodology. However, its application on the identification of mycobacteria isolates has been hampered by the structure of their cell wall. Improvements in the sample processing method and in the available database have proved key factors for the rapid and reliable identification of non-tuberculous mycobacteria isolates using MALDI-TOF MS AIMS: The main objective is to provide information about the proceedings for the identification of non-tuberculous isolates using MALDI-TOF MS and to review different sample processing methods, available databases and the interpretation of the results.&lt;/p&gt;

&lt;p&gt;&lt;b&gt;SOURCES: &lt;/b&gt;Results from relevant studies on the use of the available MALDI-TOF MS instruments, the implementation of innovative sample processing methods or the implementation of improved databases are discussed.&lt;/p&gt;

&lt;p&gt;&lt;b&gt;CONTENT: &lt;/b&gt;Insight about the methodology required for reliable identification of non-tuberculous mycobacteria and its implementation in the microbiology laboratory routine is provided.&lt;/p&gt;

&lt;p&gt;&lt;b&gt;IMPLICATIONS: &lt;/b&gt;Microbiology laboratories where MALDI-TOF MS is available can benefit from its capacity to identify most clinically interesting non-tuberculous mycobacteria in a rapid, reliable and inexpensive manner.&lt;/p&gt;
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