<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Guo, Wenbo</style></author><author><style face="normal" font="default" size="100%">Zhichen Fan</style></author><author><style face="normal" font="default" size="100%">Kai Fan</style></author><author><style face="normal" font="default" size="100%">Jiajia Meng</style></author><author><style face="normal" font="default" size="100%">Dongxia Nie</style></author><author><style face="normal" font="default" size="100%">Emmanuel Tangni</style></author><author><style face="normal" font="default" size="100%">Li, Zenghe</style></author><author><style face="normal" font="default" size="100%">Zhao, Zhihui</style></author><author><style face="normal" font="default" size="100%">Han, Zheng</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In Vivo Kinetics and Biotransformation of Aflatoxin B1 in Dairy Cows Based on the Establishment of a Reliable UHPLC-MS/MS MethodDataSheet1.docx</style></title><secondary-title><style face="normal" font="default" size="100%">Frontiers in Chemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Aflatoxins</style></keyword><keyword><style  face="normal" font="default" size="100%">biotransformation</style></keyword><keyword><style  face="normal" font="default" size="100%">dairy cow</style></keyword><keyword><style  face="normal" font="default" size="100%">in vivo kinetics</style></keyword><keyword><style  face="normal" font="default" size="100%">UHPLC-MS/MS</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Dec-12-2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">9</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The&amp;nbsp;in vivo&amp;nbsp;kinetics of aflatoxin B1&amp;nbsp;(AFB1) and its carry-over as aflatoxin M1&amp;nbsp;(AFM1) in milk as well as the toxin loads in the tissue of dairy cows were assessed through a repetitive feeding trial of an AFB1-contaminated diet of 4&amp;nbsp;μg&amp;nbsp;kg−1&amp;nbsp;body weight (b.w.) for 13&amp;nbsp;days. This was followed by a clearance period that ended with a single dose trial of an AFB1-contaminated diet of 40&amp;nbsp;μg&amp;nbsp;kg−1&amp;nbsp;b.w. An ultra-high performance liquid chromatography tandem mass spectrometry method was developed and successfully validated by the determination of linearity (R2&amp;nbsp;≥ 0.990), sensitivity (lower limit of quantification, 0.1–0.2&amp;nbsp;ng&amp;nbsp;ml−1), recovery (79.5–111.2%), and precision relative standard deviation (RSD) ≤14.7%) in plasma, milk, and various tissues. The repetitive ingestion of AFB1&amp;nbsp;indicated that the biotransformation of AFB1&amp;nbsp;to AFM1&amp;nbsp;occurred within 48&amp;nbsp;h, and the clearance period of AFM1&amp;nbsp;in milk was not more than 2&amp;nbsp;days. The carry-over rate of AFM1&amp;nbsp;in milk during the continuous ingestion experiment was in the range of 1.15–2.30% at a steady state. The&amp;nbsp;in vivo&amp;nbsp;kinetic results indicated that AFB1&amp;nbsp;reached a maximum concentration of 3.8 ± 0.9&amp;nbsp;ng&amp;nbsp;ml−1&amp;nbsp;within 35.0 ± 10.2&amp;nbsp;min and was slowly eliminated from the plasma, with a half-life time (T1/2) of 931.1 ± 30.8&amp;nbsp;min. Meanwhile, AFM1&amp;nbsp;reached a plateau in plasma (0.5 ± 0.1&amp;nbsp;ng&amp;nbsp;ml−1) at 4&amp;nbsp;h after the ingestion. AFB1&amp;nbsp;was found in the heart, spleen, lungs, and kidneys at concentrations of 1.6 ± 0.3, 4.1 ± 1.2, 3.3 ± 0.9 and 5.6 ± 1.4&amp;nbsp;μg&amp;nbsp;kg−1, respectively. AFM1&amp;nbsp;was observed in the spleen and kidneys at concentrations of only 0.7 ± 0.2 and 0.8 ± 0.1&amp;nbsp;μg&amp;nbsp;kg−1, respectively. In conclusion, the&amp;nbsp;in vivo&amp;nbsp;kinetics and biotransformation of AFB1&amp;nbsp;in dairy cows were determined using the developed UHPLC-MS/MS method, and the present findings could be helpful in assessing the health risks to consumers.&lt;/p&gt;
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