<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Marie-Alice Fraiture</style></author><author><style face="normal" font="default" size="100%">Andrea Gobbo</style></author><author><style face="normal" font="default" size="100%">Ugo Marchesi</style></author><author><style face="normal" font="default" size="100%">Daniela Verginelli</style></author><author><style face="normal" font="default" size="100%">N. Papazova</style></author><author><style face="normal" font="default" size="100%">Nancy Roosens</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking</style></title><secondary-title><style face="normal" font="default" size="100%">International Journal of Food Microbiology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antimicrobial resistance genes</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA walking anchored on pUB110</style></keyword><keyword><style  face="normal" font="default" size="100%">food and feed safety</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetically modified bacteria</style></keyword><keyword><style  face="normal" font="default" size="100%">Protease</style></keyword><keyword><style  face="normal" font="default" size="100%">real-time PCR detection</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2021</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2021</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">354</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A PCR-based DNA walking analysis was performed on a protease product suspected to contain a new unauthorized&amp;nbsp;genetically modified microorganism&amp;nbsp;(GMM). Though the characterization of unnatural associations of sequences between the pUB110&amp;nbsp;shuttle vector&amp;nbsp;and a&amp;nbsp;Bacillus amyloliquefaciens&amp;nbsp;gene coding for a protease, the presence of the GMM was shown. Based on these sequences of interest, a real-time PCR marker was developed to target specifically the newly discovered GMM, namely GMM protease2. The performance of the real-time PCR marker was assessed in terms of specificity and sensitivity. The applicability of the real-time PCR GMM protease2 marker was also demonstrated on microbial fermentation products. To confirm its use by other GMO enforcement laboratories, the transferability of the in-house validated real-time PCR marker was demonstrated by assays performed by an external laboratory.&lt;/p&gt;
</style></abstract><section><style face="normal" font="default" size="100%">109330</style></section></record></records></xml>