<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>13</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Surbhi Ranjan</style></author><author><style face="normal" font="default" size="100%">Sano-Magnifique Dusingizimana</style></author><author><style face="normal" font="default" size="100%">Tanika Van Mulders</style></author><author><style face="normal" font="default" size="100%">Eric Deconinck</style></author><author><style face="normal" font="default" size="100%">Erwin Adams</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Multidimensional Fingerprint Development for the Detection of Tribulus terrestris in Plant Food Supplements</style></title><secondary-title><style face="normal" font="default" size="100%">Drug Analysis</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Fingerprints</style></keyword><keyword><style  face="normal" font="default" size="100%">plant food supplements</style></keyword><keyword><style  face="normal" font="default" size="100%">Tribulus terrestris</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">13/09/2022</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">Drug Analysis</style></publisher><pub-location><style face="normal" font="default" size="100%">Mons, Belgium</style></pub-location><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Tribulus terrestris is a popular Ayurvedic herb with potency enhancing properties. According&lt;br&gt;
to the Belgian Royal Decree of 1997, Tribulus is a regulated plant when used in plant food&lt;br&gt;
supplements. The dangers of self-medication and toxicity of such plant supplements is&lt;br&gt;
heightened by the ease with which they can be purchased from various illegal channels,&lt;br&gt;
necessitating an efficient screening approach.&lt;br&gt;
This study aims to develop a multidimensional approach using chromatographic fingerprinting&lt;br&gt;
at different wavelengths for identification of Tribulus terrestris in plant food supplements. A&lt;br&gt;
specific, selective and fast fingerprinting method for Tribulus (reference material obtained from&lt;br&gt;
the American Herbal Pharmacopoeia) was developed using ultra high performance liquid&lt;br&gt;
chromatography coupled with a diode array detector. Next, a correlation analysis comparing&lt;br&gt;
the wavelengths was performed and 5 orthogonal wavelengths were selected. The developed&lt;br&gt;
method was tested for specificity in different triturations (1/20, 1/15, 1/10, 1/5 and 1/2). These&lt;br&gt;
were prepared using 10 blank matrices and lactose. In most triturations, Tribulus could visually&lt;br&gt;
be detected from concentration 1/10 onwards, by comparing the fingerprints with the reference&lt;br&gt;
plant.&lt;br&gt;
A market study of a total of 20 samples (with potency enhancement as indication) seized by&lt;br&gt;
the Belgian Federal Agency for Medicines and Health Products (FAMHP) was carried out.&lt;br&gt;
Visual inspection was conducted to detect the presence of Tribulus in the real samples. This&lt;br&gt;
was done by overlaying the sample chromatograms with the reference plant and visually&lt;br&gt;
comparing the fingerprints. So, out of 20 samples, 11 samples were suspected to be positive,&lt;br&gt;
when analyzed at the 5 wavelengths, 254 nm and with the combined fingerprint. The multi and&lt;br&gt;
combined wavelengths approach provides a characteristic and specific fingerprint with more&lt;br&gt;
information at different wavelengths than only 254 nm, which is useful for visual inspection. In&lt;br&gt;
a second step, mass spectrometric (MS) measurements using a time of flight analyzer were&lt;br&gt;
performed. This was done in order to further confirm the presence of Tribulus in suspected&lt;br&gt;
samples. After obtaining MS fingerprints, the exact mass of the main peaks (peaks with the&lt;br&gt;
highest absorbance in the reference) were compared. It was concluded that out of 11 samples,&lt;br&gt;
9 can be suspected to be positive for Tribulus terrestris. As future prospects, steps are being&lt;br&gt;
taken to combine chromatographic fingerprinting and chemometrics in order to use these&lt;br&gt;
multidimensional data and extract maximum information with the intention to create binary&lt;br&gt;
models for faster screening of plant food supplements.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">BSPS, UMons, ULB</style></issue></record></records></xml>