<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>13</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Wannes Van Hassel</style></author><author><style face="normal" font="default" size="100%">Mohamed F. Abdallah</style></author><author><style face="normal" font="default" size="100%">Maria Garcia Guzman Valesquez</style></author><author><style face="normal" font="default" size="100%">Christopher O. Miles</style></author><author><style face="normal" font="default" size="100%">Ignunn A. Samdal</style></author><author><style face="normal" font="default" size="100%">Julien Masquelier</style></author><author><style face="normal" font="default" size="100%">Mirjana Andjelkovic</style></author><author><style face="normal" font="default" size="100%">A. Rajkovic</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Accumulation-depuration potential and natural occurrence of Microcystin-LR toxin in basil</style></title><secondary-title><style face="normal" font="default" size="100%">International Association for Food Protection-Annual Meeting</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">July 223</style></date></pub-dates></dates><publisher><style face="normal" font="default" size="100%">Sciensano</style></publisher><pub-location><style face="normal" font="default" size="100%">Toronto, Canada</style></pub-location><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;a&gt;Accumulation of hepatotoxic cyanobacterial toxins, like microcystin-LR (MC-LR), in edible crops through irrigation with contaminated water can result in human health risks.&lt;/a&gt;&lt;/p&gt;

&lt;p&gt;To assess t&lt;a&gt;he accumulation and depuration potential of MC-LR in basil under an optimized laboratory condition and to quantify its natural occurrence in basil samples collected from different markets in Belgium.&lt;/a&gt;&lt;/p&gt;

&lt;p&gt;&lt;a&gt;Basil plants in hydroculture were exposed to 5, 10 or 50 µg L&lt;sup&gt;-1&lt;/sup&gt; MC-LR for 7 days. The depuration process was assessed by transferring plants to uncontaminated Hoagland solution &amp;nbsp;for another 7 days. Moreover, 50 basil products were collected from the Belgian markets. Basil leaves (lab and market) and roots (lab only) were analyzed using a validated UHPLC-MS/MS-based method to quantify MC-LR. ELISA and HRLCMS-techniques were applied to verify MC-LR presence in accumulation and depuration samples.&lt;/a&gt;&lt;a href=&quot;#_msocom_3&quot; id=&quot;_anchor_3&quot; name=&quot;_msoanchor_3&quot; uage=&quot;JavaScript&quot;&gt;[A3]&lt;/a&gt;&amp;nbsp;&lt;/p&gt;

&lt;p&gt;&lt;a&gt;Concentration dependent accumulation of MC-LR was observed in both basil leaves and roots, reaching for the highest treatment condition up to 87.90 µg kg&lt;sup&gt;-1&lt;/sup&gt; and 143.80 µg kg&lt;sup&gt;-1&lt;/sup&gt;, respectively. The basil roots accumulated more toxin compared to the leaves. Depuration was observed for all treatment conditions in both roots and leaves. At least six replicates were included and the whole experiment was repeated two times. These results were corroborated by both the ELISA and HRLCMS at the highest treatment condition. Moreover, MC-LR was detected below LOQ (1 µg kg&lt;sup&gt;-1&lt;/sup&gt;) in one market sample.&lt;/a&gt;&lt;/p&gt;

&lt;p&gt;&lt;a&gt;These results show the potential of basil to accumulate MC-LR from irrigation water, potentially resulting in human exposure to high levels of toxin. For the first time in Belgium, MC-LR was also detected in a vegetable from the market, showing human exposure through vegetables is already a reality.&lt;/a&gt;&lt;/p&gt;
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