Functional and comparative analysis of two distinct ecdysteroid-responsive gene expression constructs in Drosophila S2 cells4409

Last updated on 22-8-2019 by Anonymous (non vérifié)

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Peer reviewed scientific article

Résumé:

Inducible expression systems have proven to be of major interest when analysing the function of specific genes or when expressing cytotoxic proteins. In an effort to develop inducible switches allowing for flexible fine-tuning of gene expression levels in insect cells, we have compared the induction capacities of two Drosophila minimal promoters when linked to four consecutive ecdysone response elements. These minimal promoters, either containing a TATA-box or a downstream promoter element, drove the expression of a luciferase reporter gene. Potent induction capacities were observed with th…

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