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Knowledge of morphology is still required when identifying new amoeba isolates by molecular techniques31201

Last updated on 23-8-2019 by Anonymous (non vérifié)

Public Access

Published

Peer reviewed scientific article

DOI : http://dx.doi.org/10.1016/j.ejop.2012.01.009

Auteurs

J.F. De Jonckheere; Gryseels,S.; Eddyani,M.

Mots-clés

  1. 0
  2. 5.8S rDNA
  3. a
  4. Amoeba
  5. article
  6. AS
  7. Belgique
  8. Brussels
  9. chemistry
  10. classification
  11. Cluster Analysis
  12. cytology
  13. Base de données
  14. Databases
  15. de
  16. disease
  17. Maladies
  18. Dna
  19. DNA,Protozoan
  20. DNA,Ribosomal
  21. DNA,Ribosomal Spacer
  22. electronic
  23. environment
  24. Environmental Microbiology
  25. evidence
  26. Genes,rRNA
  27. genetics
  28. Ghana
  29. im
  30. Institute
  31. IS
  32. isolation & purification
  33. journal
  34. KNOWLEDGE
  35. Molecular
  36. Molecular Sequence Data
  37. Morphology
  38. Phylogeny
  39. Research
  40. Research Support
  41. Rna
  42. RNA,Protozoan
  43. RNA,Ribosomal,5.8S
  44. SB - IM
  45. Sequence Analysis,DNA
  46. Still
  47. strain
  48. Technique

Résumé:

We have isolated several free-living amoeba strains from the environment in Ghana, which have internal transcribed spacers, including the 5.8S rDNA, sequences similar to sequences attributed to Vahlkampfiidae (Heterolobosea) in databases. However, morphological examination shows that the isolates belong to the Hartmannellidae (Amoebozoa). We provide evidence that the sequences in the databases are wrongly classified as belonging to a genus or species of the Vahlkampfiidae, but rather belong to strains of the genus Hartmannella
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