Nucleotide polymorphism-based single-tube test for robust molecular identification of all currently described Brucella species.

Last updated on 23-8-2019 by Anonymous (non vérifié)

Peer reviewed scientific article



Among the numerous molecular methods described during the last 20 years to identify Brucella, multiplexed amplification methods offer the cheapest and simplest technical solution for molecular identification. However, one disadvantage of such methods is their need to undergo technical revalidation each time a new marker is added to the system. Moreover, polymorphic markers cannot be assessed at the single-nucleotide level in these assays. Since new Brucella species are continuously being described, open methodologies able to accommodate new markers while preserving all other system paramete…

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