Technical note: Flow cytometric identification of bovine milk neutrophils and simultaneous quantification of their viability.

Last updated on 23-8-2019 by Anonymous (non vérifié)

Peer reviewed scientific article

SCIENSANO

Résumé:

The aim of the present study was to develop a flow cytometric procedure for the quantification of the proportion of viable, apoptotic, and necrotic polymorphonuclear neutrophilic leukocytes (PMNL) isolated from both low- and high-somatic-cell-count quarter milk samples. Milk PMNL were differentiated from other cells by indirect fluorescent labeling using a primary anti-bovine granulocyte monoclonal antibody (CH138A) and an Alexa 647-labeled secondary antibody. Polymorphonuclear neutrophilic leukocytes were identified flow cytometrically based on their cytoplasmic granularity and CH138A-posi…

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