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Résultats de la recherche - 11 results
Genomic monitoring of SARS-CoV-2 variants using sentinel SARI hospital surveillance.
patients fulfilling the Belgian SARI definition between January 2020 and December 2022 were sequenced using the ARTIC Network amplicon tiling approach on a MinION platform. Weekly variant of concern (VOC) ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
on-site application. In addition to the comparison of the quality, quantity and purity of the extracted DNA, the performance obtained when doing Nanopore sequencing on a MinION flow cell was also tested. ...
Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies
a putative pathogen in the food sample. The DNA fragment harboring characteristic virulence genes could be matched to the E. coli genus after sequencing only 1 h with the MinION, 1 h with the Flongle if using ...
Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination.
Antibiotics (Basel), Volume 9, Issue 8 (2020) Keywords: Antimicrobial resistance MinION Plasmid sequencing Abstract: Antimicrobial resistance (AMR) is one of the most prominent public health threats. AMR genes ...
Targeting the 16S rRNA gene for bacterial identification in complex mixed samples: comparative evaluation of second (Illumina) and third (Oxford Nanopore Technologies) generation sequencing technologies.
could be eliminated at the genus level but not the species level. Alternatively, the entire 16S gene was amplified and sequenced using the ONT MinION, and analyzed with Mothur, EPI2ME, and GraphMap. ...
Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO.
the feasibility to integrate the described workflow with the MinION Next-Generation-Sequencing (NGS). The MinION sequencing platform can provide long read-lengths and deal with heterogenic DNA ...
Use of next‐generation sequencing in microbial risk assessment
assessment MinION MiSeq nanopore sequencing next generation sequencing Abstract: Despite the ever increase in rigorous control and monitoring measures to assure safe food along the entire farm‐to‐fork chain, ...
ST131 fimH22 Escherichia coli isolate with a blaCMY-2/IncI1/ST12 plasmid obtained from a patient with bloodstream infection: highly similar to E. coli isolates of broiler origin.
European collaborators. The plasmid from ESBL20160056 was sequenced using a MinION Mk1B (Oxford Nanopore Technologies). RESULTS: The E. coli isolate from the Danish patient clustered together with 13 other ...
Characterization of In Vivo acquired resistance of Mycoplasma hyopneumoniae to macrolides and lincosamides.
Publication Type: Peer reviewed scientific article Authors: Stakenborg, Tim; Vicca, Jo; Butaye, Patrick; Maes, Dominiek; Minion, F Chris; Johan Peeters; de Kruif, Aart; Haesebrouck, Freddy Source: ...
Resistance mechanism against fluoroquinolones in Mycoplasma hyopneumoniae field isolates.
Publication Type: Peer reviewed scientific article Authors: Vicca, J; Maes, D; Stakenborg, T; Butaye, P; Minion, F; Johan Peeters; de Kruif, A; Decostere, A; Haesebrouck, F Source: Microb Drug ...