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Résultats de la recherche - 74 results

Targeted Chromosomal Barcoding Establishes Direct Genotype-Phenotype Associations for Antibiotic Resistance in Mycobacterium abscessus.

Gonzalo-Asensio, Jesús Source: Microbiol Spectr (2023) Abstract: A bedaquiline-resistant Mycobacterium abscessus isolate was sequenced, and a candidate mutation in the gene was identified as responsible for the ...

Development of a Taxon-Specific Real-Time Polymerase Chain Reaction Method to Detect Trichoderma reesei Contaminations in Fermentation Products

several unexpected GMM contaminations to be highlighted. Currently, only bacterial strains are targeted by this strategy. Given that fungal strains, like Trichoderma reesei, are also frequently used by the ...

Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

sequencing PCR-based enrichment single nucleotide variations Transgenic Rice Abstract: Similar to genetically modified organisms (GMO s) produced by classical genetic engineering, gene-edited (GE) organisms ...

The 2021 WHO catalogue of complex mutations associated with drug resistance: A genotypic analysis.

catalogue of mutations to serve as a global standard for interpreting molecular information for drug resistance prediction. Methods: A candidate gene approach was used to identify mutations as associated with ...

Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking

Microbiology, Volume 354 (2021) Keywords: Antimicrobial resistance genes DNA walking anchored on pUB110 food and feed safety Genetically modified bacteria Protease real-time PCR detection Abstract: A PCR-based ...

Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants

and January 2021. The lower inclusivity of the China CDC assay targeting the gene N has continued to decrease with new mismatches detected, whereas the other evaluated assays kept their inclusivity ...

Deep amplicon sequencing for culture-free prediction of susceptibility or resistance to 13 anti-tuberculous drugs.

Here, we evaluated the Deeplex Myc- TB targeted deep sequencing assay for prediction of resistance to 13 anti-tuberculous drugs/drug classes, directly applicable on sputum. With MTBC DNA tests, the limit ...

Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.

obtained for the assay of Chan et al. (2020) targeting the gene coding for the spiking protein (S). This demonstrates that our user-friendly strategy can be used for a first in silico specificity evaluation ...

Strategy for the identification of micro-organisms producing food and feed products: Bacteria producing food enzymes as study case

Fraiture; Nancy Roosens Source: Food Chemistry, Volume 305 (2020) Keywords: 16S-rRNA gene sequencing bacteria Food enzymes identification PCR technology Producing organisms SCREENING Abstract: Recent European ...

Detection strategy targeting a chloramphenicol resistance gene from genetically modified bacteria in food and feed products

associated recombinant DNA. Although the use of AMR genes gives rise to public health and environmental concerns regarding their potential acquisitions by pathogens and gut microbiota, no method targeting AMR ...

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