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Résultats de la recherche - 167 results

Development of a reverse transcriptase digital droplet polymerase chain reaction‐based approach for SARS‐CoV‐2 variant surveillance in wastewater

Publication Type: Peer reviewed scientific article Authors: Laura Van Poelvoorde; Andrea Gobbo; S. Nauwelaerts; Bavo Verhaegen; Marie Lesenfants; Raphael Janssens; Veronik Hutse; Marie-Alice Fraiture; Sigrid C.J. De Keersmaecker; Philippe Herman; Koenraad ...

Validation of the Flemish-Dutch diabetic foot ulcer scale short form (DFS-SF) questionnaire for diabetic foot ulcers

the multidisciplinary diabetic foot clinic of Onze-Lieve-Vrouw Hospital Aalst, Belgium. DFS- SF items were reverse-coded so that high DFS- SF scores indicate better quality of life. Reliability was ...

Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants.

existing reverse transcriptase real-time PCR (RT-qPCR) assay, we developed a reverse transcriptase droplet digital PCR assay (RT-ddPCR) to detect and quantify the frequency of the E119V- NA mutation. ...

Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants

Publication Type: Peer reviewed scientific article Authors: Laura Van Poelvoorde *; François Dufrasne *; Steven Van Gucht *; Xavier Saelens; Nancy Roosens * Source: Current Issues in Molecular Biology, Volume 45, Issue 3 (2023) Health Topics:  Health and ...

Redesign and Validation of a Real-Time RT-PCR to Improve Surveillance for Avian Influenza Viruses of the H9 Subtype.

Calogero; Monne, Isabella Source: Viruses, Volume 14, Issue 6 (2022) Keywords: Animals Humans Influenza A virus Influenza in Birds Poultry Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase ...

Orbivirus Screening from Imported Captive Oryx in the United Arab Emirates Stresses the Importance of Pre-Import and Transit Measures

about 4000 SHO s from two locations in the UAE were blood sampled to be tested by indirect enzyme-linked immunosorbent assay (ELISA) and real-time reverse transcriptase polymerase chain reaction (RT-qPCR) ...

Strategy to Develop and Evaluate a Multiplex RT-ddPCR in Response to SARS-CoV-2 Genomic Evolution.

such as specificity, sensitivity and applicability. Therefore, a duplex reverse-transcription droplet digital PCR (RT-ddPCR) method was evaluated in silico by using 154 489 whole-genome sequences of ...

Assessment of an application on a detoxification process of groundnut press cake for aflatoxins by ammoniation

that the process is reproducible and reliable and to demonstrate that the detoxification is not reversible. In addition, genotoxicity testing of extracts of the treated feedingstuff and of the identified ...

Reverse thinking: taking a healthy diet perspective towards food systems transformations

Publication Type: Peer reviewed scientific article Authors: I. D. Brouwer; M. J. van Liere; A. de Brauw; P. Dominguez-Salas; A. Herforth; G. Kennedy; C. Lachat; E. B. Omosa; E. F. Talsma; Stefanie Vandevijvere; J. Fanzo; M. Ruel Source: Food Security, Vol ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses

early diagnosis, and therefore fast and adequate detection, is needed. To this end, a multiplex reverse transcription real-time polymerase chain reaction TaqMan method was designed to detect Zika (ZIKV) ...

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