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Résultats de la recherche - 15 results
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms
propose a targeted high-throughput sequencing approach, including (i) a prior PCR-based enrichment step to amplify regions of interest, (ii) a sequencing step, and (iii) a data analysis methodology to ...
Evaluation of a commercial exogenous internal process control for diagnostic RNA virus metagenomics from different animal clinical samples
Virological Methods, Volume 283 (2020) Keywords: Animals Feces High-Throughput Nucleotide Sequencing Mengovirus Metagenomics Rna RNA Virus Infections RNA Viruses Swine Swine Diseases Viral Sensitivity and ...
Targeting the 16S rRNA gene for bacterial identification in complex mixed samples: comparative evaluation of second (Illumina) and third (Oxford Nanopore Technologies) generation sequencing technologies.
Keersmaecker; Kevin Vanneste Source: Int J Mol Sci, Volume 21, Issue 1 (2019) Keywords: bacteria DNA, Bacterial High-Throughput Nucleotide Sequencing Nanopores RNA, Ribosomal, 16S Abstract: Rapid, accurate ...
Complete coding sequence of a novel picorna-like virus in a blackbird infected with Usutu virus.
Picornaviridae Infections Plants whole genome sequencing Abstract: Using random high-throughput RNA sequencing, the complete coding sequence of a novel picorna-like virus (a 9,228-nt contig containing 212,202 ...
Next-generation sequencing workflows in veterinary infection biology: towards validation and quality assurance.
High-Throughput Nucleotide Sequencing Nucleic Acid Amplification Techniques Quality Control Reproducibility of Results Workflow Abstract: Recent advancements in DNA sequencing methodologies and sequence data ...
Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin.
Volume 15, p.103 (2015) Keywords: Bacillus subtilis genetic engineering High-Throughput Nucleotide Sequencing Organisms, Genetically Modified Real-Time Polymerase Chain Reaction riboflavin Sensitivity and ...
Small RNA deep sequencing identifies viral microRNAs during malignant catarrhal fever induced by alcelaphine herpesvirus 1.
Benjamin G Source: J Gen Virol, Volume 96, Issue 11, p.3360-72 (2015) Keywords: Animals Cattle Cattle Diseases Gammaherpesvirinae Herpesviridae Infections High-Throughput Nucleotide Sequencing Malignant ...
Next-generation sequencing shows West Nile virus quasispecies diversification after a single passage in a carrion crow (Corvus corone) in vivo infection model.
10, p.2999-3009 (2015) Keywords: Animals Chickens Crows Disease Models, Animal Genetic Variation High-Throughput Nucleotide Sequencing Molecular Sequence Data Reverse Transcription RNA, Viral Sequence ...
Next-generation sequencing in veterinary medicine: how can the massive amount of information arising from high-throughput technologies improve diagnosis, control, and management of infectious diseases?
Richard; Rosseel, Toon Source: Methods Mol Biol, Volume 1247, p.415-36 (2015) Keywords: Animal Diseases Animals Communicable Diseases Disease Management High-Throughput Nucleotide Sequencing Veterinary ...
The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing.
Volume 8, Issue 9, p.e76144 (2013) Keywords: Avulavirus bias DNA Primers DNA, Complementary High-Throughput Nucleotide Sequencing Nucleic Acid Amplification Techniques virology Abstract: Sequence ...