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Résultats de la recherche - 76 results
Belgian Mosquitoes Are Competent for Japanese Encephalitis Virus and Readily Feed on Pigs, Suggesting a High Vectorial Capacity.
to seven-day-old F0-generation adult mosquitoes, emerged from field-collected larvae, were fed on a JEV genotype 3 Nakayama strain spiked blood meal. Blood-fed mosquitoes were subsequently incubated ...
Development and Validation of a New DIVA Real-Time PCR Allowing to Differentiate Wild-Type Lumpy Skin Disease Virus Strains, Including the Asian Recombinant Strains, from Neethling-Based Vaccine Strains.
difficulties to existing DIVA PCR tests, as these do not differentiate between homologous vaccine strains and the recombinant strains. We, therefore, developed and validated a new duplex real-time PCR capable of ...
Introduction of WGS in food microbiology: advantages and challenges
the field of bacterial food safety investigation, due to its high discriminatory power and the general use at the international level. Therefore, the Scientific Committee advises the FASFC to gradually ...
The history of foot-and-mouth disease virus serotype C: the first known extinct serotype?Abstract
between 1957 and 2004. Many serotype C viruses from European and Kenyan outbreaks were closely related to vaccine strains, including the most recently recovered Kenyan isolate from 2004. International ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
conventional methods in e.g. the field of pathogen detection/characterization along the food chain, starting from the food production environment (including detection of AMR genes) until foodborne outbreak ...
Molecular and virological characterization of the first poultry outbreaks of Genotype VII.2 velogenic avian orthoavulavirus type 1 (NDV) in North-West Europe, BeNeLux, 2018.
polybasic sequence of the fusion gene cleavage site and the intracerebral pathotyping assay demonstrated the high pathogenicity of the strain. This paper represents the first notification of this specific ...
Validation of TaqMan-Based Assays for Specific Detection and Differentiation of Wild-Type and Neethling Vaccine Strains of LSDV.
Petrović; Ilse De Leeuw; Andy Haegeman Source: Microorganisms, Volume 9, Issue 6 (2021) Keywords: differentiation DIVA diagnostic protocol field strain Lumpy skin disease virus Real-time PCR vaccine strain ...
A molecular assay for rapidly distinguishing the AviPro SALMONELLA VAC T vaccine strain from wild-type field isolates
genes which enable to distinguish field and vaccine strains. Based on this information, we developed and validated (n = 199) a Luminex-based assay targeting seven specific single-nucleotide polymorphisms. ...
Overview of diagnostic tools for Capripox virus infections.
purpose. The need for high throughput, high specificity/sensitivity and the capability for differentiating field virus strains from vaccine strains drives the development of new and better assays preferably ...
Development of a genoserotyping system for the identification of Salmonella serotypes
adapted to the need of the field, especially for the professionals of the food sector who need to rapidly, accurately and cost-efficiently detect the serotypes targeted by the legislation. Fortunately, ...