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Résultats de la recherche - 46 results

Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

sequencing PCR-based enrichment single nucleotide variations Transgenic Rice Abstract: Similar to genetically modified organisms (GMO s) produced by classical genetic engineering, gene-edited (GE) organisms ...

Metagenomic Characterization of Multiple Genetically Modified Bacillus Contaminations in Commercial Microbial Fermentation Products

transgenic constructs were characterized, with insertions of alpha-amylase genes originating from B. amyloliquefaciens and B. licheniformis, respectively, and a transgenic construct with a protease gene ...

Lentiviral vector induces high-quality memory T cells via dendritic cells transduction.

Lentivirus mice Mice, Inbred C57BL Promoter Regions, Genetic Transduction, Genetic Transgenes Abstract: We report a lentiviral vector harboring the human β2-microglobulin promoter, with predominant expression ...

Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO.

region. This DNA walking is performed on transgenic elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this ...

Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

of interest, including their transgene flanking regions and the unnatural associations of elements in their transgenic cassettes. To this end, the p35S, tNOS and t35S pCAMBIA elements have been ...

An integrated strategy combining DNA walking and NGS to detect GMO

strategy in routine GMO analysis. In all tested samples, the presence of multiple GMO s was unambiguously proven by the characterization of transgene flanking regions and the combinations of elements that ...

Scientific opinion on Malachite green in food

and/or mice, and of micronuclei in mice. LMG also induced cII transgene mutations in mouse liver. MG caused a small, not dose-related, increase in thyroid gland follicular adenomas and carcinomas, and of ...

Field trials with GM trees: a step-by-step approach

Publication Type: Scientific book or chapter Authors: D. C.M. Glandorf; Didier Breyer Source: Biosafety of forest transgenic trees- improving the scientific basis for safe tree development and ...

Strategies to detect unauthorized GMO in the food and feed chain (PhD thesis)

alia on elements found in their transgenic cassette. Second, according to this information, key targets, frequently found in GMO (p35S and tNOS) or exclusively observed in EU unauthorized GMO (t35S ...

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