Evaluation of 16 commercial antibody ELISAs for the detection of bovine viral diarrhea virus-specific antibodies in serum and milk using well-characterized sample panels. [1]
Peer reviewed scientific article
SCIENSANO
Auteurs
Jean-Baptiste Hanon [2]; Miet De Baere [3]; De la Ferté, Camille [4]; Sophie Roelandt [5]; Yves Van der Stede [6]; Ann Brigitte Cay [7]Mots-clés
Résumé:
We performed a thorough fit-for-purpose evaluation of commercial ELISAs for the detection of bovine viral diarrhea virus (BVDV)-specific antibodies in serum and in milk by testing 2 panels of well-characterized serum and milk samples. Sixteen ELISAs from 9 different manufacturers, available on the Belgian market at the time of our study, were assessed for their diagnostic and analytical sensitivity (DSe and ASe, respectively), diagnostic specificity (DSp), and repeatability relative to the virus neutralization (VN) test considered to be the gold standard assay. Using serum as a matrix, DSe …